Rehabilitation Radiology Department, Beijing Rehabilitation Hospital, Capital Medical University, Beijing 100144, China.
Biomed Res Int. 2022 Aug 30;2022:6790665. doi: 10.1155/2022/6790665. eCollection 2022.
Since the early 1980s when MRI imaging technology was put into clinical use, the number of MRI clinical tests has steadily increased by more than 10% every year. At the same time, exogenous MRI contrast agents have also been developed with the development of MRI technology. However, there are still challenges in the preparation of contrast agents for magnetic resonance imaging, such as how to prepare high-efficiency contrast agents with high stability and low biological toxicity. In order to study the contrast agent with simple preparation method, low cost, and good imaging effect, a magnetic resonance contrast agent was prepared by magnetic nanoparticles. By acting on magnetic resonance imaging detection method, and using polymer ligands to synthesize magnetic nanoparticles, experiments and tests of P(MA-alt-VAc) polymer ligand-modified magnetic nanoparticles were carried out. The experimental results showed that when nanoparticles containing different iron ion concentrations were incubated with DC 2.4 normal cells for 48 hours, the cell viability was still higher than 80% at concentrations up to 200 m. It shows that the nanoparticle has high cell activity and good biological adaptability. The transverse relaxation ( ) value of the nanoparticles in aqueous solution at 37°C and 1.5 T magnetic field is 231.1 m s, which is much higher than that of PTMP-PMAA ( = 35.1 mM s), which is also more than five times the relaxation of SHU-555C ( = 44 mM s). It shows that the nanoparticles prepared in this paper have good effect and can be used as a contrast agent in human brain for magnetic resonance imaging.
自 20 世纪 80 年代 MRI 成像技术投入临床使用以来，MRI 临床检测数量每年以超过 10%的速度稳步增长。与此同时，随着 MRI 技术的发展，外源性 MRI 对比剂也得到了发展。然而，在磁共振成像对比剂的制备方面仍然存在挑战，例如如何制备具有高稳定性和低生物毒性的高效对比剂。为了研究具有简单制备方法、低成本和良好成像效果的造影剂，本文通过磁共振成像检测方法作用，采用聚合物配体合成磁性纳米粒子，对 P(MA-alt-VAc)聚合物配体修饰的磁性纳米粒子进行了实验和测试。实验结果表明，当含有不同铁离子浓度的纳米粒子与 DC 2.4 正常细胞孵育 48 小时时，在浓度高达 200 m 时，细胞活力仍高于 80%。这表明纳米粒子具有高细胞活性和良好的生物适应性。在 37°C 和 1.5 T 磁场下，纳米粒子在水溶液中的横向弛豫率（ ）为 231.1 m s，明显高于 PTMP-PMAA（ = 35.1 mms），也比 SHU-555C（ = 44 mms）的弛豫率高 5 倍以上。这表明本文制备的纳米粒子具有良好的效果，可以作为磁共振成像人脑中的造影剂。
