Ma Tianying, Ran Tianfei, Ke Song, Qin Yinyin, Wang Min
Department of Orthopaedics, Second Affiliated Hospital of Army Medical University, PLA, Chongqing, China.
Evid Based Complement Alternat Med. 2022 Aug 30;2022:5463383. doi: 10.1155/2022/5463383. eCollection 2022.
The purpose of this study was to reveal the antibacterial activity of carbon-based nanofilm titanium alloy.
The experiments were performed both in vitro and in vivo of animals using two circular-piece test specimens of the same specification, which were made from carbon-based nanofilm coated titanium alloy and commonly used in artificial joints Co-Cr-Mo alloy, respectively. In the in vitro experiments, the two test specimens were cocultured with standard strains of and , and the antibacterial properties of the two test specimens were evaluated via inhibition zone size, scanning electron microscopy, fluorescence staining, colony forming unit count, and others; the cytotoxicities of the two test specimens were evaluated by coculturing and them with rabbit bone marrow mesenchymal stem cells (BMSCs). In the in vivo experiments, the two test specimens were implanted in the muscle tissue of experimental rabbits to evaluate their histocompatibilities.
Both in vitro cocultures of the carbon-based nanofilm titanium alloy and the Co-Cr-Mo alloy with and failed to form inhibition zone. However, some biofilms were observed on the surface of the Co-Cr-Mo alloy. Fewer bacteria adhered to the carbon-based nanofilm titanium alloy can be observed via scanning electron microscopy and fluorescence staining techniques. Meanwhile, the colony forming counts showed that, compared with the Co-Cr-Mo alloy, the carbon-based nanofilm titanium alloy had fewer bacteria adhered ( < 0.05). After coculture of the two test specimens with rabbit BMSCs, there was no significant difference in cell count, and both cell counts showed no cytotoxicity. In the in vivo experiment of animals, there are relatively fewer giant cells and better histocompatibility in tissues near the carbon-based nanofilm titanium alloy.
Compared with Co-Cr-Mo alloy, the novel carbon-based nanofilm titanium alloy enjoys stronger in vitro antibacterial activity and better in vivo histocompatibility.
本研究旨在揭示碳基纳米膜钛合金的抗菌活性。
使用分别由碳基纳米膜涂层钛合金和人工关节常用的钴铬钼合金制成的两个相同规格的圆形试件，在动物体外和体内进行实验。在体外实验中，将两个试件分别与标准菌株和进行共培养，通过抑菌圈大小、扫描电子显微镜、荧光染色、菌落形成单位计数等评估两个试件的抗菌性能；通过与兔骨髓间充质干细胞（BMSCs）共培养评估两个试件的细胞毒性。在体内实验中，将两个试件植入实验兔的肌肉组织中评估其组织相容性。
碳基纳米膜钛合金和钴铬钼合金与和的体外共培养均未形成抑菌圈。然而，在钴铬钼合金表面观察到一些生物膜。通过扫描电子显微镜和荧光染色技术可观察到附着在碳基纳米膜钛合金上的细菌较少。同时，菌落形成计数显示，与钴铬钼合金相比，碳基纳米膜钛合金附着的细菌较少（<0.05）。两个试件与兔BMSCs共培养后，细胞计数无显著差异，且两种细胞计数均未显示细胞毒性。在动物体内实验中，碳基纳米膜钛合金附近组织中的巨细胞相对较少，组织相容性较好。
与钴铬钼合金相比，新型碳基纳米膜钛合金具有更强的体外抗菌活性和更好的体内组织相容性。
