CEACAM6 可作为肺腺癌脑膜转移的生物标志物。
CEACAM6 serves as a biomarker for leptomeningeal metastasis in lung adenocarcinoma.
机构信息
Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Central Laboratory, Huashan Hospital, Fudan University, Shanghai, China.
出版信息
Cancer Med. 2023 Feb;12(4):4521-4529. doi: 10.1002/cam4.5221. Epub 2022 Sep 9.
BACKGROUND AND AIMS
Diagnosis of leptomeningeal metastasis (LM) is challenging. In our previous study, CEACAM6 mRNA was found to be highly expressed in the circulating tumor cells of cerebrospinal fluid (CSF) from patients with lung adenocarcinoma with LM (LUAD-LM). The aim of this study was to identify whether CEACAM6 could be used as a biomarker for LUAD-LM.
MATERIALS AND METHODS
The level of CEACAM6 was determined by enzyme-linked immunosorbent assay (ELISA) in CSF from 40 LUAD-LM and 44 normal controls, and additional serum samples from 138 LUAD patients, including 12 LUAD-LM patients, and 30 healthy controls. Carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA 21-1) and neuron-specific enolase (NSE) levels in the CSF and sera were detected by chemiluminescent immunoassay. Receiver operating characteristic curve was plotted to evaluate the diagnostic performance for LUAD-LM.
RESULTS
CSF CEACAM6 level was higher in LUAD-LM than that in normal controls. In serum, LUAD patients had a higher level of CAECAM6 than healthy controls, and LM patients had the highest level among them. Serum CEACAM6 had a higher AUC than CEA in differentiating LM from non-LM in LUAD patients (0.95 vs. 0.64, p < 0.001).
CONCLUSION
CEACAM6 may serve as a potential biomarker in diagnosing LUAD-LM.
背景与目的
脑膜转移（LM）的诊断具有挑战性。在我们之前的研究中，发现 CEACAM6mRNA 在伴有 LM 的肺腺癌（LUAD-LM）患者的脑脊液（CSF）循环肿瘤细胞中高度表达。本研究旨在确定 CEACAM6 是否可作为 LUAD-LM 的生物标志物。
材料与方法
采用酶联免疫吸附试验（ELISA）检测 40 例 LUAD-LM 和 44 例正常对照者 CSF 中 CEACAM6 水平，并检测 138 例 LUAD 患者（包括 12 例 LUAD-LM 患者和 30 例健康对照者）的额外血清样本。采用化学发光免疫分析法检测 CSF 和血清中的癌胚抗原（CEA）、细胞角蛋白 19 片段（CYFRA21-1）和神经元特异性烯醇化酶（NSE）水平。绘制受试者工作特征曲线以评估 LUAD-LM 的诊断性能。
结果
与正常对照组相比，LUAD-LM 患者 CSF 中 CEACAM6 水平更高。在血清中，LUAD 患者的 CEACAM6 水平高于健康对照者，而 LM 患者的 CEACAM6 水平最高。在 LUAD 患者中，血清 CEACAM6 在区分 LM 与非 LM 方面的 AUC 高于 CEA（0.95 比 0.64，p<0.001）。
结论
CEACAM6 可能作为诊断 LUAD-LM 的潜在生物标志物。
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