WuXi AppTec, Planegg, Germany.
Methods Mol Biol. 2022;2541:195-205. doi: 10.1007/978-1-0716-2545-3_23.
DNA-encoded library (DEL) screens are used to discover novel chemical matter capable of modulating the activity of pharmaceutically interesting protein targets. DEL selections are accomplished by immobilizing a target protein on a resin and capturing library molecules that bind to the target. The barcodes of the captured library molecules are then amplified and sequenced. This chapter outlines simple methods for visualizing the resulting screening data (using free open-source software), such that enriched molecules can be selected for synthesis and follow-up activity confirmation. Measures of enrichment and the concept of sub-libraries are also illustrated.
DNA 编码文库 (DEL) 筛选用于发现能够调节有药用价值的蛋白质靶标活性的新型化学物质。DEL 选择是通过将靶蛋白固定在树脂上并捕获与靶标结合的文库分子来完成的。然后对捕获的文库分子的条形码进行扩增和测序。本章概述了可视化筛选结果数据的简单方法（使用免费的开源软件），以便选择富集的分子进行合成和后续活性确认。还说明了富集的度量和子文库的概念。
