Department of Chromatography, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Sklodowska University in Lublin, Lublin, Poland.
Crit Rev Anal Chem. 2024;54(6):1502-1524. doi: 10.1080/10408347.2022.2121599. Epub 2022 Sep 9.
Vitamin K is one of the many health-promoting substances whose impact on the human body has been underestimated until recently. However, recently published research results have changed this situation, prompting some researchers to consider it a new panacea for diseases of old age. The result is a significant increase in interest in the accurate analysis of vitamin K in various types of samples, ranging from food, through dietary supplements, to biological matrices and clinical trials, both observational and interventional. This review summarizes the current state of knowledge about the proven and speculated biological activity of vitamin K and its importance for the world's aging societies, including the methods used for its isolation and analysis in various matrices types. Of all the analytical methods, the currently preferred methods of choice for the direct analysis of vitamin K are chromatographic methods, in particular liquid chromatography-tandem mass spectrometry. This technique, despite its sensitivity and selectivity, requires an appropriate stage of sample preparation. As there is still room for improvement in the efficiency of these methods, especially at the sample preparation stage, this review shows the directions that need to be taken to make these methods faster, more efficient and more environmentally friendly.
维生素 K 是众多促进健康的物质之一，其对人体的影响直到最近才被低估。然而，最近发表的研究结果改变了这种情况，促使一些研究人员将其视为治疗老年疾病的新灵丹妙药。其结果是，人们对各种类型样本中维生素 K 的准确分析产生了浓厚的兴趣，这些样本包括食物、膳食补充剂、生物基质和临床试验，包括观察性和干预性研究。本文综述了目前关于维生素 K 的已证实和推测的生物活性及其对世界老龄化社会的重要性的知识现状，包括在各种基质类型中用于其分离和分析的方法。在所有分析方法中，目前首选用于维生素 K 直接分析的方法是色谱法，特别是液相色谱-串联质谱法。尽管该技术具有灵敏度和选择性，但需要适当的样品制备阶段。由于这些方法的效率，尤其是在样品制备阶段，仍有改进的空间，因此本文综述了需要采取的方向，以使这些方法更快、更高效、更环保。
