ALAB Laboratories, Research and Development Centre, ul. Ceramiczna 1, 20-150 Lublin, Poland.
Department of Chromatography, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Skłodowska University in Lublin, Pl. Maria Curie-Skłodowska 3, 20-031 Lublin, Poland.
Molecules. 2023 Sep 8;28(18):6523. doi: 10.3390/molecules28186523.
Biological properties of menaquinone-7, one of the vitamin K2 vitamers (K2MK-7), both those proven and those that remain to be investigated, arouse extensive interest that goes beyond the strictly scientific framework. The most important of them is the prevention of age-related diseases, considering that we live in the times identified as the era of aging societies and many people are exposed to the vitamin K2MK-7 deficiency. Therefore, an effective analytical protocol that can be adopted as a diagnostic and preventive analytics tool is needed. Herein, a simple sample preparation method followed by the liquid chromatography-tandem mass spectrometry-based method (LC-MS/MS), was used for the selective and sensitive determination of K2MK-7 in serum samples. Under the optimized conditions, using 500 µL of serum and the same amount of -hexane, the reproducibility and the accuracy were obtained in the ranges of 89-97% and 86-110%, respectively, and the limit of detection value was 0.01 ng/mL. This method was used for the routine analysis. Statistical interpretation of the data from 518 samples obtained during 2 years of practice allowed for obtaining information on the content and distribution of K2MK-7 in the Polish population, broken down by the sex and age groups.
维生素 K2 同型物（K2MK-7）之一的menaquinone-7 的生物学特性，无论是已证实的还是有待研究的，都引起了广泛的关注，这种关注超出了严格的科学框架。其中最重要的是预防与年龄相关的疾病，因为我们生活在被确定为老龄化社会时代，许多人都面临着维生素 K2MK-7 缺乏的问题。因此，需要一种有效的分析方案，可以将其作为诊断和预防分析工具。在此，采用了一种简单的样品制备方法，随后采用基于液相色谱-串联质谱法（LC-MS/MS）的方法，用于选择性和灵敏地测定血清样品中的 K2MK-7。在优化条件下，使用 500 µL 血清和相同量的正己烷，可以分别在 89-97%和 86-110%的范围内获得重现性和准确性，检测限为 0.01 ng/mL。该方法用于常规分析。对 2 年实践中获得的 518 个样本的数据进行统计解释，使我们能够获得波兰人群中 K2MK-7 的含量和分布信息，按性别和年龄组进行了细分。
