Department of Chromatography, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Skłodowska University in Lublin, pl. Maria Curie-Skłodowska 3, 20-031 Lublin, Poland.
Research and Development Centre, ALAB Laboratories, ul. Ceramiczna 1, 20-150 Lublin, Poland.
Molecules. 2024 Sep 17;29(18):4420. doi: 10.3390/molecules29184420.
The health-promoting properties of vitamin K stimulate the growing interest in this compound, which translates into the development of new analytical methodologies for its determination. New, more efficient methods of its isolation are sought, paying increasingly more attention to the methods within currently available extraction techniques that, owing to the optimization of the process, not only increase the extraction efficiency but are also economical and environmentally friendly. This article proposes a procedure for the extraction and analysis of one of the vitamin K vitamers, i.e., vitamin K1, using PLE and LC-MS/MS. It has been shown that the PLE technique can be optimized with a mathematical model-accelerating and reducing the costs of the extraction process-which, together with process automation, bodes well for industrial applications. The optimized process was used to extract vitamin K1 from various vegetables, showing very different contents of the test compound ranging from 1.22 to 114.30 µg/g dry weight for avocado and spinach, respectively. In addition, by showing the effect of water within the material subjected to extraction on the variable yield of vitamin K1, attention was drawn to the need to standardize the analytical methods used in assessing the quality of food products.
维生素 K 的促进健康特性激发了人们对这种化合物的浓厚兴趣，这促使人们开发出了新的分析方法来对其进行定量分析。人们正在寻找更有效的分离方法，同时越来越关注目前可用的提取技术中的方法，这些方法通过对工艺的优化，不仅提高了提取效率，而且在经济和环保方面也具有优势。本文提出了一种使用加压溶剂萃取（PLE）和 LC-MS/MS 对维生素 K 同系物之一，即维生素 K1 进行提取和分析的方法。研究表明，通过数学模型可以对 PLE 技术进行优化——加速并降低萃取过程的成本——这与工艺自动化相结合，为工业应用提供了良好的前景。该优化工艺用于从各种蔬菜中提取维生素 K1，从鳄梨和菠菜中分别获得了测试化合物的含量为 1.22-114.30 µg/g 干重，这一含量差异非常大。此外，通过展示萃取材料中水分对维生素 K1 可变产率的影响，引起了人们对标准化分析方法在评估食品质量中的必要性的关注。
