Anhui Province Key Laboratory of Integrated Pest Management on Crops, Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.
School of Life Sciences, Chongqing University, Chongqing, 401331, China.
Dev Comp Immunol. 2023 Jan;138:104530. doi: 10.1016/j.dci.2022.104530. Epub 2022 Sep 6.
The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is one of the most highly polyphagous invasive pests causing serious damage to maize crops in China. However, little is known about the gut immune responses to the environment, particularly along the migration routes in Jianghuai, China, throughout the autumn and winter. In this study, high-throughput sequencing and real-time quantitative PCR (RT-qPCR) were employed to examine the variations in immune genes and gut microbiome communities between captive and wild fall armyworm populations. Results showed that the diversity and community of the gut's microbes were higher in wild populations, and the average weighted UniFrac distance between bacterial taxa varied. A wide variety of immune genes were more abundant in the wild populations than in others. Results indicated that diets and different survival conditions impacted the gut microbiota and immune system of S. frugiperda, which was crucial for environmental adaptation. These differences in gut microbiota and immune responses between wild and captive Fall armyworms are critical for comprehending the symbiotic relationship between microbes, immune genes, and hosts. They also highlight the need for increased focus on developing more effective and environmentally friendly pest control methods.
秋粘虫（FAW），也称草地贪夜蛾，是一种高度多食性的入侵害虫，对中国的玉米作物造成严重损害。然而，人们对其肠道的免疫反应知之甚少，特别是在中国江淮地区的秋、冬两季的迁徙过程中。在本研究中，我们采用高通量测序和实时定量 PCR（RT-qPCR）技术，研究了人工饲养和野生秋粘虫种群之间肠道免疫基因和微生物群落的变化。结果表明，野生种群的肠道微生物多样性和群落更为丰富，细菌分类群之间的平均加权 UniFrac 距离也有所不同。大量的免疫基因在野生种群中更为丰富。结果表明，饮食和不同的生存条件会影响 S. frugiperda 的肠道微生物群和免疫系统，这对环境适应至关重要。这些野生和人工饲养的秋粘虫在肠道微生物群和免疫反应方面的差异，对于理解微生物、免疫基因和宿主之间的共生关系至关重要。它们还强调了需要更加关注开发更有效和环保的害虫控制方法。
