Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs/Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, MARA/Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin 300191, China.
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs/Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, MARA/Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin 300191, China; Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences/Laboratory of Agricultural Products Processing Quality and Safety Risk Evaluation, Ministry of Agriculture and Rural Affairs, Zhanjiang, Guandong 524001, China.
Sci Total Environ. 2023 Jan 10;855:158587. doi: 10.1016/j.scitotenv.2022.158587. Epub 2022 Sep 7.
To explore the responses of soil microbial communities to concentration gradients of antibiotic residues in soil, 32 soil samples were collected from a typical greenhouse vegetable production base in Northern China in 2019. The total concentrations of 26 antibiotic residues in these soil samples was 83.24-4237.93 μg·kg, of which metabolites of tetracyclines were 23.34-1798.80 μg·kg. The total concentrations in 32 samples were clustered into three levels (L: <100 μg·kg, M: 100-300 μg·kg, H: >300 μg·kg) to elucidate the impacts of antibiotic residues on the diversity, structure, composition, function and antibiotic resistome of soil microbial community. Results showed that higher concentration of antibiotic residues in soil was prone to decrease the diversity and shift the structure and composition of soil microbial community. Antibiotic resistome occurred in soils with antibiotic residues exceeding 300 μg·kg. Interactions among soil bacteria followed the order of H > L > M, consistent with the relative abundances of mobile genetic elements. Bacteroidetes and Firmicutes were the top attributors impacting the profile of antibiotics in soil. According to weighted comprehensive pollution index of risk quotient, in 28.1 % of soil samples the residual antibiotics presented high ecological risk, whereas in the rest of soil samples the ecological risk is medium. The results will enrich the database and provide references for antibiotic contamination control in soils of the region and alike.
为了探索土壤微生物群落对土壤中抗生素残留浓度梯度的响应，于 2019 年从中国北方典型的温室蔬菜生产基地采集了 32 个土壤样本。这些土壤样本中 26 种抗生素残留的总浓度为 83.24-4237.93μg·kg，其中四环素类抗生素的代谢物浓度为 23.34-1798.80μg·kg。将 32 个样本的总浓度聚类为三个水平（L：<100μg·kg，M：100-300μg·kg，H：>300μg·kg），以阐明抗生素残留对土壤微生物群落多样性、结构、组成、功能和抗生素抗性组的影响。结果表明，土壤中抗生素残留浓度较高时，土壤微生物群落的多样性降低，结构和组成发生变化。土壤中抗生素残留超过 300μg·kg 时出现抗生素抗性组。土壤细菌之间的相互作用顺序为 H > L > M，与移动遗传元件的相对丰度一致。拟杆菌门和厚壁菌门是影响土壤中抗生素特征的主要类群。根据风险商的加权综合污染指数，在 28.1%的土壤样本中，残留抗生素呈现高生态风险，而在其余土壤样本中，生态风险为中。研究结果将丰富该地区及类似地区土壤抗生素污染控制的数据库，并提供参考。
