School of Advanced Manufacturing, Fuzhou University, Quanzhou 362000, China.
School of Resources and Environmental Science, Quanzhou Normal University, Quanzhou 362000, China.
Int J Environ Res Public Health. 2023 Feb 10;20(4):3137. doi: 10.3390/ijerph20043137.
The constant application of manure-based fertilizers in vegetable farms leads to antibiotic residue accumulation in soils, which has become a major stressor affecting agroecosystem stability. The present study investigated the adaptation profiles of rhizosphere microbial communities in different vegetable farms to multiple residual antibiotics. Multiple antibiotics, including trimethoprim, sulfonamides, quinolones, tetracyclines, macrolides, lincomycins, and chloramphenicols, were detected in the vegetable farms; the dominant antibiotic (trimethoprim) had a maximum concentration of 36.7 ng/g. Quinolones and tetracyclines were the most prevalent antibiotics in the vegetable farms. The five most abundant phyla in soil samples were , , , and , while the five most abundant phyla in root samples were , , , and . Macrolides were significantly correlated with microbial community composition changes in soil samples, while sulfonamides were significantly correlated with microbial community composition changes in root samples. Soil properties (total carbon and nitrogen contents and pH) influenced the shifts in microbial communities in rhizosphere soils and roots. This study provides evidence that low residual antibiotic levels in vegetable farms can shift microbial community structures, potentially affecting agroecosystem stability. However, the degree to which the shift occurs could be regulated by environmental factors, such as soil nutrient conditions.
在蔬菜农场中持续施用粪肥基肥料会导致土壤中抗生素残留的积累，这已成为影响农业生态系统稳定性的主要胁迫因素。本研究调查了不同蔬菜农场根际微生物群落对多种残留抗生素的适应情况。在蔬菜农场中检测到多种抗生素，包括甲氧苄啶、磺胺类、喹诺酮类、四环素类、大环内酯类、林可霉素类和氯霉素；占主导地位的抗生素（甲氧苄啶）的最大浓度为 36.7ng/g。喹诺酮类和四环素类是蔬菜农场中最常见的抗生素。土壤样本中最丰富的五个门是 、 、 、 和 ，而根样本中最丰富的五个门是 、 、 、 和 。大环内酯类与土壤样本中微生物群落组成的变化显著相关，而磺胺类与根样本中微生物群落组成的变化显著相关。土壤性质（总碳和氮含量以及 pH 值）影响根际土壤和根中微生物群落的变化。本研究提供的证据表明，蔬菜农场中低残留抗生素水平会改变微生物群落结构，可能影响农业生态系统的稳定性。然而，这种变化的程度可能受到环境因素（如土壤养分状况）的调节。
