基于网络药理学的决明子治疗动脉粥样硬化的药理作用：荟萃分析。
Pharmacological effects of the Cassia Seed on atherosclerosis: A meta-analysis based on network pharmacology.
机构信息
Department of Physiology, College of Medicine, Nanchang University, Nanchang, China.
Department of Surgery, Fuzhou Medical College, Nanchang University, Jiangxi, Fuzhou, China.
出版信息
Medicine (Baltimore). 2022 Sep 9;101(36):e30411. doi: 10.1097/MD.0000000000030411.
BACKGROUND
The aim of this study was to shed light on the active ingredients and potential targets of Cassia Seed about anti-atherosclerosis based on network pharmacology.
METHODS
The active ingredients and potential targets of Cassia Seed were obtained from traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) and SwissTargetPrediction database. Then, atherosclerosis-related targets were screened via GeneCards, online mendelian inheritance in man, therapeutic target database and DrugBank database. The common targets and protein-protein interaction (PPI) network was later identified and built. Furthermore, we used the database for annotation, visualization and integrated discovery (DAVID) database server to accomplish the enrichment analysis. The compounds-targets-pathways network was ultimately constructed by Cytoscape.
RESULTS
A total of 14 active ingredients and 475 related targets were sifted from Cassia Seed. Among 574 potential atherosclerotic targets, there were 99 targets overlapped with those of Cassia Seed. Topological analysis with Cytoscape revealed that proto-oncogene tyrosine-protein kinase proto-oncogene tyrosine-protein kinase Src, transcription factor AP-1 (JUN), mitogen-activated protein kinase 8 (MAPK8), mitogen-activated protein kinase 14 (MAPK14) and catenin beta-1 were considered as the hub gene. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis suggested that the Cassia Seed had the potential to influence varieties of biological processes and pathways, including positive regulation of smooth muscle cell proliferation, inflammatory response, tumor necrosis factor (TNF) signaling pathway, vascular endothelial growth factor (VEGF) signaling pathway and arachidonic acid (ARA) metabolism.
CONCLUSION
Taken together, our findings support that anti-atherosclerosis effects of Cassia Seed are characterized by multi-component, multi-target and multi-path mechanism of action.
背景
本研究旨在基于网络药理学探讨决明子防治动脉粥样硬化的活性成分及潜在靶点。
方法
从中药系统药理学数据库与分析平台（TCMSP）和瑞士TARGETPREDICTION 数据库中获取决明子的活性成分和潜在靶点，然后通过基因卡片（GeneCards）、在线孟德尔遗传人类在线数据库（OMIM）、治疗靶点数据库（Therapeutic Target Database, TTD）和药物银行（DrugBank）数据库筛选出与动脉粥样硬化相关的靶点。然后，鉴定并构建共同靶点和蛋白质-蛋白质相互作用（protein-protein interaction, PPI）网络。最后，使用数据库注释、可视化和综合发现（DAVID）数据库服务器进行富集分析，通过 Cytoscape 构建化合物-靶点-通路网络。
结果
从决明子中筛选出 14 种活性成分和 475 个相关靶点。在 574 个潜在的动脉粥样硬化靶点中，有 99 个靶点与决明子重叠。Cytoscape 的拓扑分析显示，原癌基因酪氨酸蛋白激酶 Src、转录因子 AP-1（JUN）、丝裂原活化蛋白激酶 8（MAPK8）、丝裂原活化蛋白激酶 14（MAPK14）和连环蛋白β-1 被认为是枢纽基因。GO 和 KEGG 富集分析表明，决明子可能影响多种生物学过程和途径，包括平滑肌细胞增殖的正调控、炎症反应、肿瘤坏死因子（TNF）信号通路、血管内皮生长因子（VEGF）信号通路和花生四烯酸（ARA）代谢。
结论
综上所述，本研究结果支持决明子防治动脉粥样硬化的作用机制具有多成分、多靶点和多途径的特点。
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