Agricultural Research Council, Animal Production, Germplasm Conservation and Reproductive Biotechnologies, Pretoria, South Africa.
Department of Animal Science, Tshwane University of Technology, Pretoria, South Africa.
Reprod Domest Anim. 2022 Dec;57(12):1644-1648. doi: 10.1111/rda.14256. Epub 2022 Sep 16.
The study aimed to determine the efficacy of different semen extenders and in vitro storage periods on the Windsnyer boar sperm quality equilibrated at 18°C. Ejaculates (n = 18) from three Windsnyer boars of known fertility were evaluated for macroscopic traits, pooled, and then allocated for extension in Beltsville Thawing Solution (BTS) and Kobidil extender. Diluted semen was stored at 18°C and evaluated at 0, 4, 8, 12, 24 and 48 h post collection for sperm microscopic traits. Semen diluted with BTS extender maintained the highest live normal sperm percentage at 4 (63.0 ± 10), 8 (49.6 ± 8.7), 12 (38.0 ± 12.6), 24 (22.6 ± 10.7) and 48 (12.0 ± 10.7) hours, when stored at 18°C (p < .05). The BTS extender maintained the sperm total motility greater than 40% up to 24 h at 18°C as compared to the other semen Kobidil extender treatments (p < .05). Average <5% of sperm abnormalities were recorded over 48 h of in vitro boars semen storage irrespective of the semen extenders. Windsnyer boars sperm quality was maintained over 48 h when the semen was diluted with BTS and stored at 18°C.
本研究旨在确定不同精液稀释液和体外储存时间对在 18°C 下平衡的 Windsnyer 公猪精子质量的影响。对三头已知生育力的 Windsnyer 公猪的精液进行了宏观特征评估，将其混合后，分配到 Beltsville 解冻液（BTS）和 Kobidil 稀释液中进行扩展。稀释后的精液在 18°C 下储存，并在收集后 0、4、8、12、24 和 48 小时进行精子微观特征评估。用 BTS 稀释液稀释的精液在 4（63.0±10）、8（49.6±8.7）、12（38.0±12.6）、24（22.6±10.7）和 48（12.0±10.7）小时时保持最高的活正常精子百分比，在 18°C 下储存时（p<0.05）。与其他精液 Kobidil 稀释液处理相比，BTS 稀释液在 18°C 下可将精子总活力保持在 40%以上长达 24 小时（p<0.05）。在体外储存 48 小时内，无论使用哪种精液稀释液，精子畸形率平均均低于 5%。当精液用 BTS 稀释并在 18°C 下储存时，Windsnyer 公猪的精子质量可维持 48 小时以上。
