Waye J S, Creeper L A, Willard H F
Chromosoma. 1987;95(3):182-8. doi: 10.1007/BF00330349.
The human alpha satellite repetitive DNA family is organized as distinct chromosomal subsets located at the centromeric regions of each human chromosome. Here, we describe a subset of the alpha satellite which is localized to human chromosome 11. The principal unit of repetition of this alpha satellite subset is an 850 bp XbaI fragment composed of five tandem diverged alphoid monomers, each approximately 171 bp in length. The pentamer repeat units are themselves tandemly reiterated, present in approximately 500 copies per chromosome 11. In filter hybridization experiments, the Alpha11 probes are specific for the centromeric alpha satellite sequences of human chromosome 11. The complete nucleotide sequences of two independent copies of the XbaI pentamer reveal a pentameric configuration shared with the alphoid repeats of chromosomes 17 and X, consistent with the existence of an ancestral pentameric repeat common to the centromeric arrays of at least these three human chromosomes.
人类α卫星重复DNA家族被组织成位于每个人类染色体着丝粒区域的不同染色体亚群。在这里，我们描述了定位于人类11号染色体的α卫星亚群。这个α卫星亚群的主要重复单元是一个850 bp的XbaI片段，由五个串联的不同α类单体组成，每个单体长度约为171 bp。五聚体重复单元本身串联重复，在每条11号染色体上约有500个拷贝。在滤膜杂交实验中，Alpha11探针对人类11号染色体的着丝粒α卫星序列具有特异性。XbaI五聚体的两个独立拷贝的完整核苷酸序列揭示了与17号染色体和X染色体的α类重复序列共有的五聚体结构，这与至少这三条人类染色体着丝粒阵列存在共同祖先五聚体重复序列相一致。
