Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
Nuffield Department of Medicine Research Building, Old Road Campus, Roosevelt Drive, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Methods Mol Biol. 2022;2574:15-30. doi: 10.1007/978-1-0716-2712-9_2.
Understanding the interactions involved during the immunological synapse between peptide, HLA-E molecules, and TCR is crucial to effectively target protective HLA-E-restricted T-cell responses in humans. Here we describe three techniques based on the generation of MHC-E/peptide complexes (MHC-E generically includes HLA-E-like molecules in human and nonhuman species, while HLA-E specifically refers to human molecules), which allow to investigate MHC-E/peptide binding at the molecular level through binding assays and by using peptide loaded HLA-E tetramers, to detect, isolate, and study peptide-specific HLA-E-restricted human T-cells.
了解免疫突触中肽、HLA-E 分子和 TCR 之间的相互作用对于有效地靶向人类保护性 HLA-E 限制的 T 细胞反应至关重要。在这里，我们描述了三种基于 MHC-E/肽复合物生成的技术（MHC-E 通常包括人类和非人类物种中的 HLA-E 样分子，而 HLA-E 则专门指人类分子），这些技术允许通过结合测定和使用负载肽的 HLA-E 四聚体在分子水平上研究 MHC-E/肽结合，以检测、分离和研究肽特异性 HLA-E 限制的人类 T 细胞。
