HLA-E/ specific CD4 and CD8 T cells have a memory phenotype in individuals with TB infection.

INTRODUCTION

Tuberculosis (TB) is the deadliest infectious disease worldwide and novel vaccines are urgently needed. HLA-E is a virtually monomorphic antigen presentation molecule and is not downregulated upon HIV co-infection. HLA-E restricted specific CD8 T cells are present in the circulation of individuals with active TB (aTB) and infection (TBI) with or without HIV co-infection, making HLA-E restricted T cells interesting vaccination targets for TB.

METHODS

Here, we performed in-depth phenotyping of HLA-E/ specific and total T cell populations in individuals with TBI and in individuals with aTB or TBI and HIV using HLA-E/ tetramers.

RESULTS AND DISCUSSION

We show that HIV co-infection is the main driver in changing the memory distribution of HLA-E/ specific CD4 and CD8 T cell subsets. HLA-E/ specific CD4 and CD8 T cells were found to circulate with comparable frequencies in all individuals and displayed expression of KLRG1, PD-1 and 2B4 similar to that of total T cells. The presence of HLA-E/ specific T cells in individuals with aTB and TBI highlights the potential of HLA-E as a vaccine target for TB.