通过 scRNA- 和 TCR-seq 技术对人类 KIRCD8 调节性 T 细胞进行特征分析。
Characterization of KIRCD8 Regulatory T Cells in Humans by scRNA- and TCR-seq.
机构信息
Institute of Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, CA, USA.
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.
出版信息
Methods Mol Biol. 2022;2574:41-121. doi: 10.1007/978-1-0716-2712-9_4.
Previous studies have demonstrated the regulatory functions of Ly49CD8 T cells toward self-reactive CD4 T cells in mice. Recently, we found KIRCD8 T cells are the equivalent of mouse Ly49CD8 T cells in humans. They are increased in patients with autoimmune or infectious diseases as a negative feedback mechanism to suppress the arising pathogenic cells and maintain peripheral tolerance. Here, we describe the methods on how we characterize the KIRCD8 T cells from different diseases using single-cell RNA and TCR sequencing.
先前的研究已经证明了 Ly49CD8 T 细胞在小鼠中对自身反应性 CD4 T 细胞的调节功能。最近，我们发现 KIRCD8 T 细胞在人类中相当于小鼠的 Ly49CD8 T 细胞。它们在自身免疫或感染性疾病患者中增加，作为一种负反馈机制来抑制出现的致病性细胞并维持外周耐受。在这里，我们描述了如何使用单细胞 RNA 和 TCR 测序来表征来自不同疾病的 KIRCD8 T 细胞的方法。
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