Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey.
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey; Ministry of Agriculture and Forestry, Veterinary Control Institute, Elazig, Turkey.
Comp Immunol Microbiol Infect Dis. 2022 Oct;89:101867. doi: 10.1016/j.cimid.2022.101867. Epub 2022 Aug 27.
The aim of this study was to identify Neosopora caninum, Toxoplasma gondii and Tritricomonas foetus in all cattle aborted fetus samples and N. caninum and T. gondii in sheep and goat aborted fetuses sent to Elazig Veterinary Control Institute during two years. Total genomic DNAs were obtained using a commercial kit. Real-time PCR analysis was performed separately for each agent. Conventional PCR was set up for confirmation of positive samples. Then, fetal brain, heart, lung and liver samples were analysed by hematoxylin-eosin (HE) and Avidin-Biotin Complex (ABC) Immunohistochemistry (IHC) methods. Totally, we tested 55 aborted fetus samples. Of these samples, seven (12.7 %) was belonged to goats, 18 (32.7 %) to sheep and 30 (54.5 %) to cattle. T. gondii was detected in six (10.90 %) samples, and four (7.27 %) of them were positive with Real-time PCR, while only one of these four samples was positive for both classical PCR and IHC. N. caninum was determined by at least one of the three tests in 14 (25.45 %) of the samples studied, while 8 (14.54 %) of the positive samples were detected by Real-time PCR, only two of them were also positive with conventional PCR, eight (14.54 %) samples was determined as positive by IHC. Considering T. foetus in the samples, positivity was determined in two (3.63 %) of 55 aborted fetus (both of which were aborted cattle fetus) by Real-time PCR, while only one of them was positive with conventional PCR, while no positivity was detected with the IHC.
本研究的目的是在两年内向埃拉泽兽医控制研究所送检的所有牛流产胎儿样本中鉴定犬新孢子虫、刚地弓形虫和胎儿三毛滴虫，以及绵羊和山羊流产胎儿中的犬新孢子虫和刚地弓形虫。使用商业试剂盒提取总基因组 DNA。分别对每种病原体进行实时 PCR 分析。为确认阳性样本建立了常规 PCR。然后，通过苏木精-伊红（HE）和亲和素-生物素复合物（ABC）免疫组织化学（IHC）方法分析胎儿脑、心、肺和肝组织样本。总共测试了 55 个流产胎儿样本。其中，7 个（12.7%）属于山羊，18 个（32.7%）属于绵羊，30 个（54.5%）属于牛。在 6 个（10.90%）样本中检测到刚地弓形虫，其中 4 个（7.27%）通过实时 PCR 呈阳性，而这 4 个样本中只有 1 个同时通过经典 PCR 和 IHC 呈阳性。在研究的样本中，通过至少三种检测中的一种确定了犬新孢子虫在 14 个（25.45%）样本中存在，而在 8 个（14.54%）阳性样本中，实时 PCR 检测为阳性，只有 2 个样本常规 PCR 也为阳性，8 个（14.54%）样本通过 IHC 确定为阳性。考虑到样本中的胎儿三毛滴虫，通过实时 PCR 在 55 个流产胎儿（均为流产牛胎儿）中确定了 2 个（3.63%）为阳性，其中只有 1 个通过常规 PCR 呈阳性，而 IHC 未检测到阳性。
