Wang Yuefei, Gao Xia, Wang Jiajia, Tang Ming, Yu Boyang, Wang Zhenzhong, Cao Liang, Chen Xialin, Qian Mengyu, Wang Shanli, Xiao Wei
Jiangsu Kanion Modern Chinese Medicine Institute, Nanjing 210017, China; State Key Laboratory of Pharmaceutical New-Tech for Chinese Medicine, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China; National Enterprise Technology Center, National Post-doctoral Research Workstation, Jiangsu Enterprise Academician Workstation, Lianyungang 222001, China; China Pharmaceutical University, Nanjing 210009, China.
Jiangsu Kanion Modern Chinese Medicine Institute, Nanjing 210017, China; State Key Laboratory of Pharmaceutical New-Tech for Chinese Medicine, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China; National Enterprise Technology Center, National Post-doctoral Research Workstation, Jiangsu Enterprise Academician Workstation, Lianyungang 222001, China.
J Pharm Biomed Anal. 2022 Oct 25;220:115005. doi: 10.1016/j.jpba.2022.115005. Epub 2022 Aug 17.
Sinomenium acutum stem is widely used to treat rheumatoid arthritis, gout, ankylosing spondylitis and other diseases in China. However, its metabolism in vivo is still unclear. In this study, UPLC-Q-TOF/MS was used to analyze the main components and their metabolites in rats after oral administration of Sinomenium acutum stem extract. A total of 41 compounds were identified from the ethanol extract of Sinomenium acutum stem based on the established database and the reference substance; a total of 25 prototype components and 107 metabolites (74 phase I metabolites and 33 phase II metabolites) were speculated and identified in the plasma, urine, bile and feces of rats administered. The metabolic pathways included hydroxylation, demethylation, dehydrogenation, glucuronidation and acetylation. In conclusion, this study revealed the metabolism of Sinomenium acutum stem in vivo, which may provide a better basis for the study of Sinomenium acutum stem and provide useful chemical information on the material basis and pharmacological mechanism of drug efficay.
在中国，青风藤茎被广泛用于治疗类风湿性关节炎、痛风、强直性脊柱炎等疾病。然而，其体内代谢情况仍不清楚。本研究采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-Q-TOF/MS）分析大鼠口服青风藤茎提取物后的主要成分及其代谢产物。基于所建立的数据库和对照品，从青风藤茎乙醇提取物中鉴定出41种化合物；在给药大鼠的血浆、尿液、胆汁和粪便中推测并鉴定出25种原型成分和107种代谢产物（74种I相代谢产物和33种II相代谢产物）。代谢途径包括羟基化、去甲基化、脱氢、葡萄糖醛酸化和乙酰化。总之，本研究揭示了青风藤茎在体内的代谢情况，可为青风藤茎的研究提供更好的依据，并为药物疗效的物质基础和药理机制提供有用的化学信息。
