School of Pharmacy, Nanjing Medical University, Nanjing, China.
People's Hospital of Kizilsu Kirgiz Autonomous Prefecture, Atushi, Xinjiang, China.
Planta Med. 2024 Feb;90(2):138-153. doi: 10.1055/a-2184-1134. Epub 2023 Sep 29.
as a Kirgiz medicine is widely used for the treatment of frostbite and chronic rheumatoid arthritis. However, due to a lack of systematic research on the chemical components of and their metabolites, the bioactive components in it remain unclear. Herein, a reliable strategy based on UHPLC-Q-TOF-MS/MS was established to comprehensively analyze the chemical components and their metabolites . In total, 59 compounds were identified from stem extract, among which 14 isoflavones, 10 isoprenylated isoflavones, 4 polyhydroxy flavonoids, 9 alkaloids and 1 polyol were characterized for the first time. After oral administration of stem extract, 30 prototype constituents and 28 metabolites (12 phase I and 16 phase II metabolites) were speculated on and identified in rat serum, urine and feces. Furthermore, the metabolic pathways of the chemical components were systematically analyzed and proposed. In conclusion, the chemical components from stem and their metabolites were first studied, which may provide useful chemical information for further study on the effective material basis and pharmacological mechanism of .
作为吉尔吉斯斯坦的传统药物，被广泛用于治疗冻伤和慢性类风湿关节炎。然而，由于缺乏对其化学成分及其代谢产物的系统研究，其生物活性成分仍不清楚。本研究建立了一种基于 UHPLC-Q-TOF-MS/MS 的可靠策略，以全面分析 茎提取物中的化学成分及其代谢产物。从 茎提取物中共鉴定出 59 种化合物，其中首次鉴定出 14 种异黄酮、10 种异戊烯基异黄酮、4 种多羟基黄酮、9 种生物碱和 1 种多元醇。口服 茎提取物后，在大鼠血清、尿液和粪便中推测并鉴定出 30 种原型成分和 28 种代谢产物（12 种 I 相代谢产物和 16 种 II 相代谢产物）。此外，还系统分析并提出了化学成分的代谢途径。总之，首次研究了 茎中的化学成分及其代谢产物，这可能为进一步研究 的有效物质基础和药理机制提供了有用的化学信息。
