Institute of Evolution & Marine Biodiversity (IEMB), Ocean University of China, Qingdao, 266003, China; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China.
Laboratory of Marine Protozoan Biodiversity and Evolution, Marine College, Shandong University, Weihai, 264209, China.
Chemosphere. 2022 Dec;308(Pt 2):136362. doi: 10.1016/j.chemosphere.2022.136362. Epub 2022 Sep 7.
Microplastics are broadly used and among the most studied environmental pollutants due to their potential impacts on organisms and human health. Amphioctopus fangsiao (Cephalopoda: Octopodidae) is an important commercial species in the Pacific Northwest and is very popular among consumers owing to its rich nutritional value and fresh flavor. However, the toxic effects of microplastic exposure on A. fangsiao, including phenotypical effect and underlying molecular mechanism, remain limited. In this study, the octopus A. fangsiao were exposed to microplastics (polystyrene microplastics, Micro-PS) at concentrations of 100 and 1000 μg/L for 21 days, and then the physiological response, histopathological analysis, biomarkers of oxidative stress and glycolipid metabolism, microbiome perturbations and transcriptomic profiles in the intestines were performed. Results demonstrated that Micro-PS exposure had distinct adverse effects on the food intake of A. fangsiao. Histological analysis revealed that Micro-PS exposure has resulted in histopathological damage, thus causing early inflammation of the intestine. Oxidative stresses, metabolic disorders and microbiome perturbations were also detected in the intestine of A. fangsiao based on physiological biomarkers and microbiome analyses. Moreover, transcriptome analysis detected the differentially expressed genes (DEGs) and significantly enriched KEGG pathways in response to oxidative stress, glycolipid metabolism, DNA damage and transmembrane transport of intestinal cells, revealing distinct toxic effects at the molecular level. In summary, Micro-PS exposure has a strong impact on the intestines of A. fangsiao. For the first time, this study uses multiple approaches based on the physiological and biochemical response as well as transcriptional regulation analysis. The first assessment of the toxic impact of this species under Micro-PS exposure is also reported.
微塑料由于其对生物和人类健康的潜在影响而被广泛使用，也是目前研究最多的环境污染物之一。太平洋西北部的美洲章鱼（Cephalopoda: Octopodidae）是一种重要的商业物种，由于其丰富的营养价值和新鲜的味道，深受消费者的喜爱。然而，微塑料暴露对美洲章鱼的毒性影响，包括表型效应和潜在的分子机制，仍然有限。在这项研究中，章鱼 A. fangsiao 暴露于微塑料（聚苯乙烯微塑料，Micro-PS）浓度为 100 和 1000 μg/L 21 天，然后进行生理反应、组织病理学分析、氧化应激和糖脂代谢生物标志物、微生物组扰动和肠道转录组谱分析。结果表明，Micro-PS 暴露对 A. fangsiao 的摄食有明显的不良影响。组织学分析显示，Micro-PS 暴露导致组织病理学损伤，从而导致肠道早期炎症。根据生理生物标志物和微生物组分析，还检测到氧化应激、代谢紊乱和微生物组扰动在 A. fangsiao 的肠道中。此外，转录组分析检测到肠道细胞中氧化应激、糖脂代谢、DNA 损伤和跨膜转运的差异表达基因（DEGs）和显著富集的 KEGG 途径，揭示了分子水平上的明显毒性作用。总之，Micro-PS 暴露对 A. fangsiao 的肠道有很强的影响。本研究首次采用基于生理生化反应和转录调控分析的多种方法，首次报道了该物种在 Micro-PS 暴露下的毒性影响评估。
