Liu Xiumei, Yang Jianmin, Li Zan
College of Life Sciences, Yantai University, Yantai, China.
School of Agriculture, Ludong University, Yantai, China.
Front Physiol. 2023 Aug 8;14:1250513. doi: 10.3389/fphys.2023.1250513. eCollection 2023.
Nanoplastics (NPs), as a new type of pollutant with a size small than 1 μm, are ubiquitous and harmful to organisms. There has been an increasing amount of research concerning the effects of NPs on organisms over recent years, especially on aquatic animals. However, there is a limited study on the impact of NPs on mollusk cephalopods. In this research, , belonging to Cephalopoda, Coleoidea, Sepioidea, was selected to explore the effects caused by NPs exposure. The larvae were exposed to polystyrene NPs (PS-NPs) with diameter 50 nm (100 mg/L) for 4 h. The detection of oxidative stress biomarkers displayed an obvious increase in SOD (superoxide dismutase) activity and MDA (malondialdehyde) level. Then, RNA-Seq was performed to explore the oxidative stress response at mRNA level. The transcriptome analysis demonstrated that the expression of 2,570 genes was affected by PS-NPs. Besides, the signaling pathways of ribosome, ribosome biogenesis in eukaryotes, proteasome, and MAPK were enriched. This study not only provides novel references for understanding the mechanisms of oxidative stress response induced by NPs, but also reminds us to follow with interest the influence of acute exposure to NPs.
纳米塑料（NPs）作为一种新型污染物，其尺寸小于1微米，无处不在且对生物体有害。近年来，关于纳米塑料对生物体影响的研究越来越多，尤其是对水生动物的影响。然而，关于纳米塑料对软体头足类动物影响的研究却很有限。在本研究中，选取了属于头足纲、鞘亚纲、乌贼目的[具体物种名称未给出]来探究纳米塑料暴露所造成的影响。将[具体物种名称未给出]的幼体暴露于直径为50纳米的聚苯乙烯纳米塑料（PS-NPs）（100毫克/升）中4小时。氧化应激生物标志物的检测显示超氧化物歧化酶（SOD）活性和丙二醛（MDA）水平明显升高。然后，进行RNA测序以探究mRNA水平的氧化应激反应。转录组分析表明，2570个基因的表达受到PS-NPs的影响。此外，核糖体、真核生物核糖体生物合成、蛋白酶体和丝裂原活化蛋白激酶的信号通路也得到了富集。本研究不仅为理解纳米塑料诱导的氧化应激反应机制提供了新的参考，也提醒我们要关注纳米塑料急性暴露的影响。
