Department of Avian Diseases, Kimron Veterinary Institute, Beit Dagan 5025001, Israel; Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.
Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.
J Virol Methods. 2022 Dec;310:114613. doi: 10.1016/j.jviromet.2022.114613. Epub 2022 Sep 7.
Avian reovirus (ARV) is a common pathogen in chickens and other birds causing a variety of clinical symptoms such as arthritis and tenosynovitis but also enteric and respiratory symptoms. A rapid method that detects as many ARV genotypes as possible, will contribute to the early identification and control of the virus infection that causes high economic damage to the poultry industry worldwide. In this study, a real-time reverse transcription polymerase chain reaction (RT-qPCR) assay for the detection of ARV was developed. The RT-qPCR detection threshold for ARV genomic RNA standard cases was 10 copies/µL. Reproducibility of the RT-qPCR was confirmed by intra- and inter-assays. When the nucleic acids of different ARV genotypes and other common avian pathogens (IBDV, AIV, NDV, and IBV) were subjected to that RT-qPCR test, only ARV samples tested positive while all other pathogens tested negative. Due to the simplicity, convenience, high sensitivity, and specificity of the assay, the probe-based RT-qPCR is proposed to be used as an alternative diagnostic assay for the detection of ARVs in veterinary diagnostic laboratories.
禽呼肠孤病毒（ARV）是一种常见的鸡和其他鸟类病原体，可引起多种临床症状，如关节炎和腱鞘炎，但也有肠道和呼吸道症状。一种能够尽可能多地检测到 ARV 基因型的快速方法，将有助于早期识别和控制这种病毒感染，这种感染会给全球家禽业造成巨大的经济损失。本研究开发了一种用于检测 ARV 的实时逆转录聚合酶链反应（RT-qPCR）检测方法。ARV 基因组 RNA 标准病例的 RT-qPCR 检测阈值为 10 拷贝/µL。通过内和间试验证实了 RT-qPCR 的重现性。当不同 ARV 基因型和其他常见禽病原体（IBDV、AIV、NDV 和 IBV）的核酸进行该 RT-qPCR 检测时，只有 ARV 样本呈阳性，而所有其他病原体均呈阴性。由于该检测方法简单、方便、灵敏度高、特异性强，建议将基于探针的 RT-qPCR 用作兽医诊断实验室中检测 ARV 的替代诊断检测方法。
