Zhang Xinning, Wang Sushi, Li Xuran, Li Xin, Ran Weizheng, Liu Chengwei, Tian Wenyang, Yu Xiaohan, Wu Chunlei, Li Pengjie, Li Nannan, Wei Yifo, Wang Yao, Yu Shun, Chen Zhigang
Department of Neurology, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6 Fangxingyuan 1st Block, Fengtai District, Beijing 100078, China China.
Department of Neurobiology, Xuanwu Hospital of Capital Medical University, 45 Changchun Street, Xicheng District, Beijing 100053, China.
Neurosci Lett. 2022 Oct 15;789:136868. doi: 10.1016/j.neulet.2022.136868. Epub 2022 Sep 7.
Previous studies have shown that α-synuclein (α-syn) accumulation in the normal aging brain is associated with a parallel increase in hemoglobin-binding α-syn (Hb-α-syn) in the brain and peripheral erythrocytes (ERCs), indicating that Hb-α-syn levels in ERCs may reflect the α-syn changes in the brain. However, if there is any change in ERC Hb-α-syn levels in disease condition is unclear. In this study, Hb-α-syn levels in ERCs from 149 Patients with multiple system atrophy (MSA) and 149 healthy controls (HCs) were measured by enzyme linked immunosorbent assay (ELISA). The results showed that Hb-α-syn levels in ERCs were significantly increased in MSA patients in comparison with those in HCs (777.84 ± 240.82 ng/mg vs 508.84 ± 162.57 ng/mg, P < 0.001). Receiver operating characteristic curve (ROC) indicated that increased Hb-α-syn in ERCs could discriminate MSA patients from HCs, with a sensitivity of 71.8%, a specificity of 80.5%, and an area under the curve (AUC) of 0.837. The positive and negative predictive values at a cut-off value of 616.12 ng/mg were 78.7% and 74.1%, respectively. However, the increase in Hb-α-syn levels did not show any association with the age of onset and consultation, disease duration, and UMSARS (I-IV) score. This pilot study suggests that ERC Hb-α-syn is increased in MSA patients and could evaluate α-syn accumulation in the brain of patients.
以往研究表明，正常衰老大脑中α-突触核蛋白（α-syn）的积累与大脑和外周红细胞（ERC）中血红蛋白结合α-突触核蛋白（Hb-α-syn）的平行增加有关，这表明ERC中的Hb-α-syn水平可能反映大脑中α-syn的变化。然而，疾病状态下ERC中Hb-α-syn水平是否有任何变化尚不清楚。在本研究中，采用酶联免疫吸附测定（ELISA）测量了149例多系统萎缩（MSA）患者和149例健康对照（HC）的ERC中Hb-α-syn水平。结果显示，与HC相比，MSA患者ERC中的Hb-α-syn水平显著升高（777.84±240.82 ng/mg对508.84±162.57 ng/mg，P<0.001）。受试者工作特征曲线（ROC）表明，ERC中Hb-α-syn升高可区分MSA患者和HC，敏感性为71.8%，特异性为80.5%，曲线下面积（AUC）为0.837。截断值为616.12 ng/mg时的阳性和阴性预测值分别为78.7%和74.1%。然而，Hb-α-syn水平的升高与发病年龄、就诊年龄、病程和UMSARS（I-IV）评分均无关联。这项初步研究表明，MSA患者的ERC中Hb-α-syn升高，可评估患者大脑中α-syn的积累情况。
