College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
School of Mechatronical Engineering, Beijing Institute of Technology, Beijing, 100081, China.
Biotechnol Lett. 2022 Nov;44(11):1301-1311. doi: 10.1007/s10529-022-03294-3. Epub 2022 Sep 10.
Cell density detection is usually the counting of cells in certain volume of liquid, which is an important process in biological and medical fields. The Coulter counting method is an important method for biological cell detection and counting. In this paper, a microfluidic chip based on two electrode pairs is designed, which uses the Coulter principle to detect the flow rate of liquid and count the cells, and then calculate the cell density. When the cell passes through the sensor channel formed by the electrode pair on the chip, the impedance will change between the electrodes. This phenomenon has been proved by experiments. The designed chip has the advantages of simple structure, small size and low manufacturing cost. The cell density detection method proposed in this article is of great significance to the research in the field of biological cell detection and development of related medical devices.
细胞密度检测通常是对一定体积液体中的细胞进行计数，这是生物和医学领域的重要过程。库尔特计数法是一种用于生物细胞检测和计数的重要方法。本文设计了一种基于两对电极的微流控芯片，该芯片利用库尔特原理检测液体流速并对细胞进行计数，进而计算出细胞密度。当细胞通过芯片上电极对形成的传感器通道时，电极之间的阻抗会发生变化。这一现象已通过实验得到证实。所设计的芯片具有结构简单、体积小、制造成本低等优点。本文提出的细胞密度检测方法对生物细胞检测领域的研究和相关医疗设备的发展具有重要意义。
