College of Horticulture, China Agricultural University, Beijing 100193, China.
Beijing Agricultural Technology Extension Station, Beijing 100029, China.
Plant Sci. 2022 Nov;324:111454. doi: 10.1016/j.plantsci.2022.111454. Epub 2022 Sep 8.
Tubby-like protein (TLP) plays an important role in plant growth and development. In this investigation, the characteristics of 11 members in the SlTLP family were studied. SlTLP genes were classified into two subgroups, and the members containing the F-box domain were renamed SlTLFPs. Subcellular localization indicated that most of the SlTLPs were localized in the nucleus. Expression pattern analysis revealed that eight genes (SlTLFP1, 3, 5, 7-10, and SlTLP11) showed differential expression across various tissues, while SlTLFP2, 4, and 6 were widely expressed in all the organs tested. Most SlTLP genes were induced by biotic and abiotic stress treatments such as Botrytis cinerea, temperature, MeJA, and ABA. TLP proteins in tomato have no transcriptional activation activity, and most members with an F-box domain could interact with SUPPRESSOR OF KINETOCHORE PROTEIN 1 (SlSkp1) or Cullin1 (Cul1) or both. Experiments on CRISPR edited SlTLFP8 showed that the N-terminal F-box domain was necessary for its function such as DNA ploidy and stomata size regulation. Our findings suggested that the F-box domain interacts with Skp1 and Cul1 to form the SCF complex, suggesting that SlTLFPs, at least SlTLFP8, function mainly through the F-box domain as an E3 ligase.
类圆体蛋白（TLP）在植物生长发育中发挥着重要作用。本研究对 SlTLP 家族的 11 个成员的特征进行了研究。SlTLP 基因被分为两个亚组，含有 F-box 结构域的成员被重新命名为 SlTLFPs。亚细胞定位表明，大多数 SlTLPs 定位于细胞核中。表达模式分析表明，8 个基因（SlTLFP1、3、5、7-10 和 SlTLP11）在不同组织中表现出差异表达，而 SlTLFP2、4 和 6 在所有测试的器官中广泛表达。大多数 SlTLP 基因受到生物和非生物胁迫处理的诱导，如 Botrytis cinerea、温度、MeJA 和 ABA。番茄中的 TLP 蛋白没有转录激活活性，大多数具有 F-box 结构域的成员可以与 SUPPRESSOR OF KINETOCHORE PROTEIN 1（SlSkp1）或 Cullin1（Cul1）或两者相互作用。对 CRISPR 编辑的 SlTLFP8 的实验表明，N 端 F-box 结构域对于其功能（如 DNA 倍性和气孔大小调节）是必需的。我们的研究结果表明，F-box 结构域与 Skp1 和 Cul1 相互作用形成 SCF 复合物，这表明 SlTLFPs，至少 SlTLFP8，主要通过 F-box 结构域作为 E3 连接酶发挥作用。
