农用抗生素克菌丹对小鼠骨髓细胞和睾丸细胞的细胞遗传学效应。

Cytogenetic effects of an agricultural antibiotic, captan, on mouse bone marrow and testicular cells.

作者信息

Feng J Y, Lin B Y

出版信息

Environ Res. 1987 Aug;43(2):359-63. doi: 10.1016/s0013-9351(87)80036-1.

Abstract

This paper deals with the cytogenetic effects of Captan on mouse bone marrow and testis. We used (a) the assays of micronuclei (MN) in polychromatic erythrocytes (PCE), (b) the mouse chromosomal aberration test on bone marrow and testis, and (c) the assay of sperm morphology. The tested doses in these assays were 1000, 800, 400, 100, and 50 mg/kg. The results showed that Captan was remarkably mutagenic to bone marrow, the lowest dose inducing MN being 100 mg/kg, and to chromosome breakage, 400 mg/kg. It was also demonstrated that captan was mutagenic to cells of the mouse testis; there was a certain relationship between dosage and response. The lowest doses inducing mutagenicity of spermatocyte and primary spermatogonia and sperm-head abnormality were 50, 800, and 200 mg/kg, respectively.

摘要

本文探讨了克菌丹对小鼠骨髓和睾丸的细胞遗传学效应。我们采用了以下方法：（a）多染性红细胞（PCE）中的微核（MN）检测；（b）小鼠骨髓和睾丸的染色体畸变试验；（c）精子形态检测。这些试验中的受试剂量分别为1000、800、400、100和50 mg/kg。结果表明，克菌丹对骨髓具有显著的致突变性，诱导微核的最低剂量为100 mg/kg，对染色体断裂的最低剂量为400 mg/kg。还证明了克菌丹对小鼠睾丸细胞具有致突变性；剂量与反应之间存在一定关系。诱导精母细胞、初级精原细胞和精子头部异常致突变性的最低剂量分别为50、800和200 mg/kg。