Tates A D, Natarajan A T
Mutat Res. 1976 Nov;37(2-3):267-77. doi: 10.1016/0027-5107(76)90041-5.
Cytogenetic damage induced by a wide range of concentrations of CNU-ethanol in mice was evaluated by determining the frequencies of (a) micro-nuclei in polychromatic erythrocytes of the bone marrow, (b) chromatid aberrations in bone marrow, (c) chromatid aberrations in spermatogonia, and (d) reciprocal translocations induced in spermatogonia and scored in spermatocytes. For CNU-ethanol the following order of sensitivity was found between the tests performed: micronuclei greater than aberrations in bone marrow greaer than aberrations in spermatogonia greater than translocations in spermatocytes. Correlation coefficients were calculated for the first three parameters. Positive correlations existed (a) between micronuclei in polychromatic erythrocytes and chromatid aberrations in bone marrow on the first day after treatment, and (b) between chromatid aberrations in bone marrow and spermatogonia at the first day after treatment. Three reciprocal translocations were induced in spermatogonia and recorded in primary spermatocytes; all were of a rare type, namely between an X chromosome and an autosome.
通过测定以下各项的频率，评估了不同浓度的环磷酰胺 - 乙醇对小鼠细胞遗传学损伤的影响：（a）骨髓多染性红细胞中的微核频率；（b）骨髓中的染色单体畸变；（c）精原细胞中的染色单体畸变；（d）精原细胞中诱导并在精母细胞中计分的相互易位。对于环磷酰胺 - 乙醇，在所进行的测试之间发现了以下敏感性顺序：微核大于骨髓中的畸变大于精原细胞中的畸变大于精母细胞中的易位。计算了前三个参数的相关系数。在治疗后第一天，（a）多染性红细胞中的微核与骨髓中的染色单体畸变之间存在正相关，（b）骨髓中的染色单体畸变与精原细胞中的染色单体畸变之间存在正相关。在精原细胞中诱导出三个相互易位，并在初级精母细胞中记录下来；所有这些都是罕见类型，即在一条X染色体和一条常染色体之间。
