Tates A D, Natarajan A T, De Vogel N, Meijers M
Mutat Res. 1977 Jul;44(1):87-95. doi: 10.1016/0027-5107(77)90117-8.
Cytogenetic damage induced by various concentrations of BCNU was evaluated by determining the frequencies of (a) micronuclei in polychromatic erythrocytes of bone marrow, (b) chromatid aberrations in bone marrow, (c) chromatid aberrations in spermatogonia, and (d) reciprocal translocations induced in spermatogonia and scored in spermatocytes. The order of sensitivity for the four parameters tested was: micronuclei greater than chromatid aberrations in bone marrow greater than aberrations in spermatogonia greater than translocations in spermatocytes, a situation similar to that found in an earlier study with CNU-ethanol. When the effect of concentration of the chemical was taken into consideration there was no correlation among the four parameters tested, so that information on induced frequencies of one parameter does not have predictive value for the frequencies of the others. A comparison of the results obtained with the bifunctional BCNU and the mono-functional CNU-ethanol at equal concentrations indicated that BCNU had a similar or a lesser clastogenic effect than did CNU-ethanol. In an experiment in vitro the situation was different in that BCNU was more effective than CNU-ethanol.
(a) 骨髓多染红细胞中的微核频率；(b) 骨髓中的染色单体畸变频率；(c) 精原细胞中的染色单体畸变频率；以及 (d) 精原细胞中诱导产生并在精母细胞中计分的相互易位频率。所测试的四个参数的敏感性顺序为：微核大于骨髓中的染色单体畸变大于精原细胞中的畸变大于精母细胞中的易位，这种情况与早期使用环己亚硝脲 - 乙醇的研究结果相似。当考虑化学物质浓度的影响时，所测试的四个参数之间没有相关性，因此一个参数的诱导频率信息对其他参数的频率没有预测价值。在相同浓度下，对双功能卡莫司汀和单功能环己亚硝脲 - 乙醇所得结果进行比较表明，卡莫司汀的致断裂效应与环己亚硝脲 - 乙醇相似或更小。在一项体外实验中，情况有所不同，卡莫司汀比环己亚硝脲 - 乙醇更有效。
