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采用逆流色谱法从 ssp. 中分离二氢异香豆素和二氢二苯乙烯类化合物。

Separation of Dihydro-Isocoumarins and Dihydro-Stilbenoids from ssp. by Use of Counter-Current Chromatography.

机构信息

Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstraße 20, 38106 Braunschweig, Germany.

Symrise AG, Mühlenfeldstraße 1, 37603 Holzminden, Germany.

出版信息

Molecules. 2022 May 26;27(11):3424. doi: 10.3390/molecules27113424.

DOI:10.3390/molecules27113424
PMID:35684362
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9182509/
Abstract

Previously, different ssp. cultivars were investigated by untargeted LC-MS analysis. From this, a list of tentatively identified and unknown compounds that differ significantly between these cultivars was obtained. Due to the lack of reference compounds, especially for dihydro-isocoumarins, we aimed to isolate and structurally characterise these compounds from the cultivar 'Yae-no-amacha' using NMR and LC-MS methods. For purification and isolation, counter-current chromatography was used in combination with reversed-phase preparative HPLC as an orthogonal and enhanced purification workflow. Thirteen dihydro-isocoumarins in combination with other metabolites could be isolated and structurally identified. Particularly interesting was the clarification of dihydrostilbenoid glycosides, which were described for the first time in ssp. . These results will help us in further studies on the biological interpretation of our data.

摘要

先前，不同的 ssp. 品种通过非靶向 LC-MS 分析进行了研究。由此获得了一份在这些品种之间差异显著的暂定鉴定和未知化合物的列表。由于缺乏参考化合物，特别是对于二氢异香豆素，我们旨在使用 NMR 和 LC-MS 方法从品种 'Yae-no-amacha' 中分离和结构表征这些化合物。为了进行纯化和分离，我们使用逆流色谱法与反相制备 HPLC 相结合，作为一种正交和增强的纯化工作流程。可以分离和结构鉴定十三种二氢异香豆素以及其他代谢产物。特别有趣的是阐明了二氢苯并二氢呋喃苷，这是在 ssp. 中首次描述的。这些结果将有助于我们进一步研究我们数据的生物学解释。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/eef9088e2e8e/molecules-27-03424-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/a81e254ca744/molecules-27-03424-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/022d4f2b639d/molecules-27-03424-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/97f806dcd7a1/molecules-27-03424-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/72e3a80ec86e/molecules-27-03424-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/56837733b950/molecules-27-03424-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/0b9b23622209/molecules-27-03424-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/5163ab13ede3/molecules-27-03424-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/049eae5d99f2/molecules-27-03424-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/eef9088e2e8e/molecules-27-03424-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/a81e254ca744/molecules-27-03424-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/022d4f2b639d/molecules-27-03424-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/97f806dcd7a1/molecules-27-03424-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/72e3a80ec86e/molecules-27-03424-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/56837733b950/molecules-27-03424-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/0b9b23622209/molecules-27-03424-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/5163ab13ede3/molecules-27-03424-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/049eae5d99f2/molecules-27-03424-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ba0/9182509/eef9088e2e8e/molecules-27-03424-g009.jpg

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2
Chemical Constituents from Leaves of Hydrangea serrata and Their Anti-photoaging Effects on UVB-Irradiated Human Fibroblasts.锯叶绣球叶的化学成分及其对紫外线B照射的人成纤维细胞的抗光老化作用
Biol Pharm Bull. 2019;42(3):424-431. doi: 10.1248/bpb.b18-00742.
3
Relative sweetness and sweetness quality of phyllodulcin [(3R)-8-Hydroxy-3-(3-hydroxy-4-methoxyphenyl)-3,4-dihydro-1H-isochromen-1-one].
异甘草素[（3R）-8-羟基-3-（3-羟基-4-甲氧基苯基）-3,4-二氢-1H-异色满-1-酮]的相对甜度和甜味品质
Food Sci Biotechnol. 2016 Aug 31;25(4):1065-1072. doi: 10.1007/s10068-016-0172-x. eCollection 2016.
4
Chemical constituents from the stems of Hydrangea paniculata.圆锥绣球茎的化学成分。
J Asian Nat Prod Res. 2017 Jun;19(6):564-571. doi: 10.1080/10286020.2017.1329829.
5
Three new coumarin glycosides from the stems of Hydrangea paniculata.圆锥绣球茎中的三种新香豆素糖苷。
J Asian Nat Prod Res. 2017 Apr;19(4):320-326. doi: 10.1080/10286020.2017.1305958.
6
New Phenylpropanoid and Coumarin Glycosides from the Stems of Hydrangea paniculata Sieb.圆锥绣球茎中的新苯丙素和香豆素糖苷
Molecules. 2017 Jan 18;22(1):133. doi: 10.3390/molecules22010133.
7
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Phytochem Anal. 2016 Mar-Apr;27(2):116-25. doi: 10.1002/pca.2606. Epub 2016 Jan 11.
8
Isolation of cytotoxic diterpenoids from the Chilean medicinal plant Azorella compacta Phil from the Atacama Desert by high-speed counter-current chromatography.通过高速逆流色谱法从智利阿塔卡马沙漠的药用植物紧密小蓬（Azorella compacta Phil）中分离细胞毒性二萜类化合物。
J Sci Food Agric. 2016 Jun;96(8):2832-8. doi: 10.1002/jsfa.7451. Epub 2015 Oct 1.
9
Monoterpenes from the leaves of Hydrangea paniculata and their hepatoprotective activities.圆锥绣球叶中的单萜类化合物及其保肝活性。
J Asian Nat Prod Res. 2015 May;17(5):512-8. doi: 10.1080/10286020.2015.1042871. Epub 2015 May 22.
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Using nonrandom two-liquid model for solvent system selection in counter-current chromatography.采用非随机双液模型进行逆流色谱中溶剂系统的选择。
J Chromatogr A. 2014 Aug 15;1355:80-5. doi: 10.1016/j.chroma.2014.05.080. Epub 2014 Jun 8.