Adeosun Wilson Bamise, More Garland K, Steenkamp Paul, Prinsloo Gerhard
Department of Agriculture and Animal Health, University of South Africa, Johannesburg, South Africa.
College of Agriculture and Environmental Sciences, University of South Africa, Johannesburg, South Africa.
Front Mol Biosci. 2022 Aug 25;9:961859. doi: 10.3389/fmolb.2022.961859. eCollection 2022.
Pharmacological studies conducted in the past revealed the potential source of medicinal plants in the development of novel medicines. The phenolic contents of medicinal plants containing chlorogenic acids (CGA) have been linked to a variety of therapeutic effects, especially antiviral activity. is a medicinal plant which has been reported to contain chlorogenic acids compounds and has also shown antiviral activities against a number of virus species including Herpes Simplex Virus-1 (HSV-1). In this study, the aim was to determine both the influence of seasonal variation and locality on the antiviral properties of . Since chlorogenic acids have been reported as potent antiviral compounds, these compounds were targeted to determine the effects of locality and seasonal change on the chlorogenic acid profile, and subsequent antiviral activity. The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectroscopy (UPLC-qTOF-MS) was employed to determine the metabolic profile variations of three derivatives of chlorogenic acids-caffeoylquinic acid (CQA), dicaffeoylquinic acid (DCQA) and tricaffeoylquinic acid (TCQA) in the harvested plants growing in two diverse geographical climates and two different seasons (spring and autumn). Using the cytopathic effect (CPE) reduction approach, twenty-six samples of the plants' leaves and stems collected during spring and autumn at Telperion nature reserve in Mpumalanga and Wakefield farm, Midlands in KwaZulu-Natal region of South Africa were evaluated for anti-HSV activity. The MTT assay was used for the cytotoxicity evaluation of the extracts prior to antiviral determination. Seventeen (mostly spring collections) of the twenty-six extracts examined were found to have considerable anti-HSV activity as measured by a reduction in tissue culture infectious dose (TCID) of less than 10. The UPLC-qTOF-MS result revealed that dicaffeoylquinic acid (DCQA) is the most abundant, with higher concentrations in both regions and seasons. 3-CQA was also shown to be the most abundant isomer of caffeoylquinic acid in this investigation.
过去进行的药理学研究揭示了药用植物在新型药物开发中的潜在来源。含有绿原酸（CGA）的药用植物中的酚类成分与多种治疗作用有关，尤其是抗病毒活性。 是一种据报道含有绿原酸化合物的药用植物，并且对包括单纯疱疹病毒1型（HSV-1）在内的多种病毒物种也显示出抗病毒活性。在本研究中，目的是确定季节变化和产地对 的抗病毒特性的影响。由于绿原酸已被报道为有效的抗病毒化合物，因此以这些化合物为目标来确定产地和季节变化对绿原酸谱以及随后的抗病毒活性的影响。采用超高效液相色谱-四极杆飞行时间质谱（UPLC-qTOF-MS）来确定在两种不同地理气候和两个不同季节（春季和秋季）生长的收获植物中绿原酸的三种衍生物——咖啡酰奎尼酸（CQA）、二咖啡酰奎尼酸（DCQA）和三咖啡酰奎尼酸（TCQA）——的代谢谱变化。使用细胞病变效应（CPE）降低法，对在南非夸祖鲁-纳塔尔省中部韦克菲尔德农场和姆普马兰加省特尔佩里翁自然保护区春季和秋季采集的26个植物叶片和茎干样本进行抗HSV活性评估。在进行抗病毒测定之前，使用MTT法对提取物进行细胞毒性评估。在所检测的26种提取物中，有17种（大多为春季采集）通过组织培养感染剂量（TCID）降低小于10来衡量，具有相当的抗HSV活性。UPLC-qTOF-MS结果显示，二咖啡酰奎尼酸（DCQA）含量最高，在两个地区和两个季节中浓度都较高。在本研究中，3-CQA也被证明是咖啡酰奎尼酸中含量最丰富的异构体。
