De Tapia M, Dietrich A, Burkard G
Eur J Biochem. 1987 Aug 3;166(3):559-63. doi: 10.1111/j.1432-1033.1987.tb13550.x.
When bean plants (Phaseolus vulgaris var. Saxa) are treated with mercuric chloride or infected with alfalfa mosaic virus, they produce pathogenesis-related (PR) proteins. We report here that functional mRNA encoding bean PR4 protein is only present when synthesis of this protein has been induced. Treatment with mercuric chloride results in a rapid induction of functional bean PR4 mRNA (within 2-3 h), whereas in virus-infected plants this mRNA can only be detected the second day following the infection. Bean PR4 protein is synthesized in vitro, using this mRNA in a rabbit reticulocyte lysate system, as a precursor of 35 kDa. This precursor can be processed into a polypeptide having the same molecular mass (33.5 kDa) as the in vivo PR4 protein by the addition to the cell-free translation system of canine pancreatic microsomal membranes.
当菜豆植株（菜豆品种Saxa）用氯化汞处理或感染苜蓿花叶病毒时，它们会产生病程相关（PR）蛋白。我们在此报告，编码菜豆PR4蛋白的功能性mRNA仅在该蛋白的合成被诱导时才存在。用氯化汞处理会迅速诱导功能性菜豆PR4 mRNA（在2 - 3小时内），而在病毒感染的植株中，这种mRNA只能在感染后的第二天检测到。利用这种mRNA在兔网织红细胞裂解物系统中体外合成菜豆PR4蛋白，其为35 kDa的前体。通过向无细胞翻译系统中添加犬胰腺微粒体膜，该前体可被加工成与体内PR4蛋白具有相同分子量（33.5 kDa）的多肽。
