Nelson S E, Loesch-Fries L S
Agrigenetics Advanced Science Company, Madison, Wisc. 53716.
Intervirology. 1987;27(3):172-6. doi: 10.1159/000149737.
cDNA of the subgenomic messenger RNA (RNA 4) of alfalfa mosaic virus was ligated in both orientations into plasmids containing the bacteriophage SP 6 promoter. Upon transcription in vitro these plasmids yielded synthetic full-length complementary RNA and messenger-sense RNA. The complementary RNA contained an open reading frame of 417 nucleotides; the messenger-sense RNA encodes the virus coat protein. No translation products from the complementary RNA were detected in a wheat germ translation system, a reticulocyte lysate translation system, or Xenopus oocytes, whereas the messenger sense RNA was very active in these translation systems.
苜蓿花叶病毒亚基因组信使核糖核酸（RNA 4）的互补脱氧核糖核酸（cDNA）以两种方向连接到含有噬菌体SP 6启动子的质粒中。在体外转录时，这些质粒产生了合成的全长互补RNA和信使义RNA。互补RNA含有一个417个核苷酸的开放阅读框；信使义RNA编码病毒外壳蛋白。在小麦胚翻译系统、网织红细胞裂解物翻译系统或非洲爪蟾卵母细胞中未检测到来自互补RNA的翻译产物，而信使义RNA在这些翻译系统中非常活跃。
