Food College, Northeast Agricultural University, Harbin, China.
Front Immunol. 2022 Aug 24;13:947755. doi: 10.3389/fimmu.2022.947755. eCollection 2022.
The intestine is the largest digestive and immune organ in the human body, with an intact intestinal mucosal barrier. is the specific gut commensals colonized in the human gut for boosting intestinal immunity to defend against intestinal mucosal immune injury. In the LPS-induced intestinal injury model, the BL-10 was suggested to boost the intestinal immune. Detailly, compared with the LPS-induced mice, the BL10 group significantly reduced intestine (jejunum, ileum, and colon) tissue injury, pro-inflammatory cytokines (TNF-α, IFN-γ, IL-2, IL-6, IL-17, IL-22, and IL-12) levels and myeloperoxidase activities. Moreover, the BL-10 significantly increased the number of immunocytes (CD4+ T cells, IgA plasma cells) and the expression of tight junction protein (Claudin1 and Occludin). BL-10 regulated the body's immune function by regulating the Th1/Th2 and Th17/Treg balance, which showed a greater impact on the Th1/Th2 balance. Moreover, the results also showed that BL-10 significantly down-regulated the intestinal protein expression of TLR4, -IκB, and NF-κB p65. The BL-10 increased the relative abundance of the genera, including NK4A136_group and UCG-014, which were related to declining the levels of intestinal injury. Overall, these results indicated that the BL-10 had great functionality in reducing LPS-induced intestinal mucosal immune injury.
肠道是人体最大的消化和免疫器官，具有完整的肠道黏膜屏障。是定植在人体肠道内的特定肠道共生菌，可增强肠道免疫，抵御肠道黏膜免疫损伤。在 LPS 诱导的肠道损伤模型中，BL-10 被认为可增强肠道免疫。详细来说，与 LPS 诱导的小鼠相比，BL10 组显著降低了肠道（空肠、回肠和结肠）组织损伤、促炎细胞因子（TNF-α、IFN-γ、IL-2、IL-6、IL-17、IL-22 和 IL-12）水平和髓过氧化物酶活性。此外，BL-10 还显著增加了免疫细胞（CD4+T 细胞、IgA 浆细胞）的数量和紧密连接蛋白（Claudin1 和 Occludin）的表达。BL-10 通过调节 Th1/Th2 和 Th17/Treg 平衡来调节机体的免疫功能，对 Th1/Th2 平衡的影响更大。此外，结果还表明，BL-10 显著下调了 TLR4、-IκB 和 NF-κB p65 的肠道蛋白表达。BL-10 增加了 NK4A136_group 和 UCG-014 等与肠道损伤水平下降相关的属的相对丰度。总体而言，这些结果表明 BL-10 具有减轻 LPS 诱导的肠道黏膜免疫损伤的巨大功能。
