Takahashi S, Asaho S, Matsuoka O
Jikken Dobutsu. 1987 Apr;36(2):177-83. doi: 10.1538/expanim1978.36.2_177.
The mean corpuscular volume (MCV) and the size distribution of circulating blood cells were determined in the fetal mice of C3H/He strain by using a new electric cell size analyser, Coulter Channelyzer C-256. On the 12th day of gestation, the volume of circulating blood cells was distributed between approximately 240-820 fl (mode at 470 fl), and the MCV was 534.9 +/- 30.1 fl, i. e., ten to eleven times that of adult one. On the 14th day, two types of cell population were observed; one with smaller cell volume and another with larger one corresponded to that of the blood cells on day 12. Therefore, two peaks were observed to be at 140 and 501 fl in the size distribution curve. The cell population with large volume observed on day 12-14 had been almost disappeared by the 16th day of gestation, and the small blood cells became dominant. The MCV of blood cells was then decreased with the development of fetus, from 188.2 +/- 19.3 fl on day 16, to 135.1 +/- 7.3 fl on day 18, and 117.5 +/- 7.2 fl on day 20. The size of blood cells continued to decrease gradually after birth, and became adult range by 8 weeks after birth. The MCV values of the blood cells were 120.9 +/- 8.6 fl, 87.5 +/- 6.2 fl, and 48.7 +/- 0.8 fl for the newborns of 1 day and 7 days old, and the adult mice, respectively. White blood cells were not separated from the blood samples in this study. However, the size distribution and MCV presented above were appeared to be related essentially to the red blood cells, since the number of white blood cells are negligible small compared with that of the red cells.
采用新型血细胞大小电分析仪库尔特通道分析仪C-256，测定了C3H/He品系胎鼠的平均红细胞体积（MCV）和循环血细胞的大小分布。妊娠第12天，循环血细胞体积分布在约240 - 820飞升之间（众数为470飞升），MCV为534.9±30.1飞升，即成年鼠的10至11倍。第14天，观察到两种细胞群体；一种细胞体积较小，另一种较大，与第12天的血细胞情况相对应。因此，在大小分布曲线上观察到两个峰值，分别位于140和501飞升处。在妊娠第12 - 14天观察到的大体积细胞群体在第16天几乎消失，小血细胞占主导。随后，随着胎儿发育，血细胞的MCV降低，从第16天的188.2±19.3飞升降至第18天的135.1±7.3飞升，第20天的117.5±7.2飞升。出生后血细胞大小继续逐渐减小，出生8周后达到成年范围。1日龄、7日龄新生鼠和成年鼠血细胞的MCV值分别为120.9±8.6飞升、87.5±6.2飞升和48.7±0.8飞升。本研究中未从血样中分离白细胞。然而，上述大小分布和MCV似乎主要与红细胞有关，因为与红细胞数量相比，白细胞数量可忽略不计。
