Fairbanks V F
Am J Clin Pathol. 1980 Jan;73(1):55-62. doi: 10.1093/ajcp/73.1.55.
Manual and automated (Coulter) methods for determining hematocrit and mean corpuscular volume (MCV) were compared using blood specimens in which normal erythrocytes had been serially diluted in their own plasma. Thus, predicted linearity of hematocrit and predicted constancy of the erythrocytic indices were evaluated by these technics. Automated hematocrit was a rectilinear function of the erythrocyte count. Centrifuged hematocrit deviated systematically from the automated hematocrit; its correlation with the erythrocyte count was curvilinear. Automated erythrocytic indices were nearly constant throughout the range of dilutions of erythrocytes. The MCV, calculated from centrifuged hemtocrit and automated erythrocyte count, varied strikingly as a function of the latter. The range of MCV so observed was 62 to 105 fl for normal blood specimens, serially diluted, which, when undiluted, had MCV values in the range of 82 to 90 fl. Results of manual (centrifuged) hematocrit and erythrocytic indices appear to be inherently less reliable than those obtained by automated methods.
使用在自身血浆中连续稀释正常红细胞的血液标本，对测定血细胞比容和平均红细胞体积（MCV）的手工方法和自动方法（库尔特法）进行了比较。因此，通过这些技术评估了血细胞比容的预测线性和红细胞指数的预测稳定性。自动血细胞比容是红细胞计数的直线函数。离心血细胞比容与自动血细胞比容存在系统性偏差；其与红细胞计数的相关性呈曲线关系。在红细胞稀释范围内，自动红细胞指数几乎保持恒定。根据离心血细胞比容和自动红细胞计数计算出的MCV，随后者的变化显著。对于连续稀释的正常血液标本，观察到的MCV范围为62至105飞升，而未稀释时其MCV值在82至90飞升范围内。手工（离心）血细胞比容和红细胞指数的结果似乎本质上不如自动方法获得的结果可靠。
