Gault M J, Gillin F D, Zenian A J
Exp Parasitol. 1987 Aug;64(1):29-37. doi: 10.1016/0014-4894(87)90005-1.
Giardia lamblia trophozoites specifically colonize the upper human small intestine which is normally serumfree but have been grown in vitro only in medium supplemented with serum or serum fractions. Recently, we demonstrated that biliary lipids will support the growth of G. lamblia without added serum. Now, we report that human duodenal jejunal mucus stimulates growth of Giardia in medium with biliary lipids. Stimulation by mucus was enhanced by inclusion of chymotrypsin or crude pancreatic proteases. Coculture of trophozoites with human intestinal epithelial cells also promoted growth, especially in the presence of mucus and/or biliary lipids. With biliary lipids alone, the mean increase in cell number was 3.2 fold and in the presence of mucus 8 fold (P less than 0.01) in 24 serial subcultures. Our demonstration that human intestinal mucus and epithelial cells promote serumfree growth of G. lamblia may help to explain specific colonization of the small intestine by G. lamblia.
蓝氏贾第鞭毛虫滋养体特异性地寄生于人类小肠上段，该部位通常无血清，但此前仅在添加了血清或血清成分的培养基中才能在体外培养。最近，我们证明胆汁脂质可在不添加血清的情况下支持蓝氏贾第鞭毛虫的生长。现在，我们报告人类十二指肠空肠黏液能在含有胆汁脂质的培养基中刺激蓝氏贾第鞭毛虫的生长。糜蛋白酶或粗制胰蛋白酶的加入可增强黏液的刺激作用。滋养体与人肠道上皮细胞共培养也能促进其生长，尤其是在有黏液和/或胆汁脂质存在的情况下。仅使用胆汁脂质时，在24次连续传代培养中，细胞数量平均增加3.2倍；在有黏液存在时，细胞数量平均增加8倍（P<0.01）。我们证明人类肠道黏液和上皮细胞可促进蓝氏贾第鞭毛虫无血清生长，这可能有助于解释蓝氏贾第鞭毛虫对小肠的特异性寄生。
