Biliary lipids support serum-free growth of Giardia lamblia.
作者信息
Gillin F D, Gault M J, Hofmann A F, Gurantz D, Sauch J F
出版信息
Infect Immun. 1986 Sep;53(3):641-5. doi: 10.1128/iai.53.3.641-645.1986.
Abstract
Giardia lamblia has been grown in vitro only in media containing serum or serum fractions. How this pathogen can grow in the human small intestinal lumen without serum is not known. We found that samples of human hepatic or gall bladder bile maintained G. lamblia survival for 24 to 48 h in medium without serum but did not support growth. By contrast, an artificial biliary lipid dispersion containing six bile salts, phosphatidylcholine (PC), and cholesterol, in the ratios characteristic of human bile, supported parasite growth in medium without serum or serum fractions. To define the requirements, we showed that 1-palmitoyl-2-linoleoyl-PC or 1-palmitoyl-2-oleoyl-PC (which predominate in human bile) satisfied the requirement for PC. Moreover, either glycocholate or glycodeoxycholate could be substituted for the bile salt mixture. The finding that biliary lipids can support serum-free growth of G. lamblia may help explain why this parasite colonizes the upper small intestine.
摘要
相似文献
1
Biliary lipids support serum-free growth of Giardia lamblia.
Infect Immun. 1986 Sep;53(3):641-5. doi: 10.1128/iai.53.3.641-645.1986.
2
Effects of bile and bile salts on growth and membrane lipid uptake by Giardia lamblia. Possible implications for pathogenesis of intestinal disease.
J Clin Invest. 1985 Nov;76(5):1727-32. doi: 10.1172/JCI112162.
3
Giardia lamblia: the role of conjugated and unconjugated bile salts in killing by human milk.
Exp Parasitol. 1987 Feb;63(1):74-83. doi: 10.1016/0014-4894(87)90080-4.
4
Giardia lamblia: stimulation of growth by human intestinal mucus and epithelial cells in serumfree medium.
Exp Parasitol. 1987 Aug;64(1):29-37. doi: 10.1016/0014-4894(87)90005-1.
5
Giardia-bile salt interactions in vitro and in vivo.
Trans R Soc Trop Med Hyg. 1988;82(3):428-32. doi: 10.1016/0035-9203(88)90153-8.
6
Mammalian bile promotes growth of Giardia lamblia in axenic culture.
Trans R Soc Trop Med Hyg. 1983;77(4):467-9. doi: 10.1016/0035-9203(83)90115-3.
7
Lipid requirements and lipid uptake by Giardia lamblia trophozoites in culture.
J Eukaryot Microbiol. 1996 May-Jun;43(3):237-42. doi: 10.1111/j.1550-7408.1996.tb01398.x.
8
Lipid analysis of Giardia lamblia and its culture medium.
Ann Trop Med Parasitol. 1988 Feb;82(1):83-90. doi: 10.1080/00034983.1988.11812213.
9
Cholesterol starvation induces differentiation of the intestinal parasite Giardia lamblia.
Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7628-33. doi: 10.1073/pnas.93.15.7628.
10
Cholesterol enhances membrane-damaging properties of model bile by increasing the intervesicular-intermixed micellar concentration of hydrophobic bile salts.
J Surg Res. 1999 Jun 1;84(1):112-9. doi: 10.1006/jsre.1999.5625.
引用本文的文献
1
Trophozoite fitness dictates the intestinal epithelial cell response to Giardia intestinalis infection.
PLoS Pathog. 2023 May 4;19(5):e1011372. doi: 10.1371/journal.ppat.1011372. eCollection 2023 May.
2
Transcriptomic Profiling of High-Density Foci Encysting in the Murine Proximal Intestine.
Front Cell Infect Microbiol. 2017 May 31;7:227. doi: 10.3389/fcimb.2017.00227. eCollection 2017.
3
Giardia Alters Commensal Microbial Diversity throughout the Murine Gut.
Infect Immun. 2017 May 23;85(6). doi: 10.1128/IAI.00948-16. Print 2017 Jun.
4
Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic La1 Inhibit Growth.
Front Microbiol. 2016 Sep 27;7:1453. doi: 10.3389/fmicb.2016.01453. eCollection 2016.
5
Sphingolipids, Lipid Rafts, and Giardial Encystation: The Show Must Go On.
Curr Trop Med Rep. 2015 Sep 1;2(3):136-143. doi: 10.1007/s40475-015-0052-0.
6
The FAD-dependent glycerol-3-phosphate dehydrogenase of Giardia duodenalis: an unconventional enzyme that interacts with the g14-3-3 and it is a target of the antitumoral compound NBDHEX.
Front Microbiol. 2015 Jun 1;6:544. doi: 10.3389/fmicb.2015.00544. eCollection 2015.
7
Lipid metabolism in Giardia: a post-genomic perspective.
Parasitology. 2011 Mar;138(3):267-78. doi: 10.1017/S0031182010001277. Epub 2010 Sep 30.
8
Clathrin-dependent pathways and the cytoskeleton network are involved in ceramide endocytosis by a parasitic protozoan, Giardia lamblia.
Int J Parasitol. 2007 Jan;37(1):21-32. doi: 10.1016/j.ijpara.2006.09.008. Epub 2006 Oct 12.
9
Uptake of [3H]-gangliosides by an intestinal protozoan, Giardia lamblia.
Parasitol Res. 2005 May;96(2):102-6. doi: 10.1007/s00436-005-1332-8. Epub 2005 Apr 6.
10
Biology of Giardia lamblia.
Clin Microbiol Rev. 2001 Jul;14(3):447-75. doi: 10.1128/CMR.14.3.447-475.2001.
本文引用的文献
1
On the concentration of bile acids in the human intestine during absorption. Bile acids and sterioids 74.
Acta Physiol Scand. 1959 Aug 31;46:339-45. doi: 10.1111/j.1748-1716.1959.tb01763.x.
2
Clonal growth of Giardia lamblia trophozoites in a semisolid agarose medium.
J Parasitol. 1980 Apr;66(2):350-2.
3
In vitro studies on bile acid deconjugation and lipolysis inhibition by Giardia lamblia.
Dig Dis Sci. 1981 Aug;26(8):700-4. doi: 10.1007/BF01316858.
4
Lipids of pigment gallstones.
Biochim Biophys Acta. 1982 Jul 20;712(1):21-5. doi: 10.1016/0005-2760(82)90079-0.
5
Chronic giardiasis: studies on drug sensitivity, toxin production, and host immune response.
Gastroenterology. 1982 Oct;83(4):797-803.
6
Enzymatic measurement of choline-containing phospholipids in bile.
J Lipid Res. 1981 Feb;22(2):373-6.
7
The influence of bile salt structure on self-association in aqueous solutions.
J Biol Chem. 1983 May 25;258(10):6362-70.
8
Lipid and carbohydrate metabolism of Giardia lamblia.
Mol Biochem Parasitol. 1981 Feb;2(3-4):187-96. doi: 10.1016/0166-6851(81)90099-2.
9
Mass cultivation of Giardia lamblia in a serum-free medium.
J Parasitol. 1983 Dec;69(6):1181-2.
10
Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile.
Trans R Soc Trop Med Hyg. 1983;77(4):487-8. doi: 10.1016/0035-9203(83)90120-7.
Zotero 插件