Cui Xiaoshuang, Liu Shangjia, Zhang Lina, Guo Xinping, Li Ting, Zhang Xiaoyu, Wang Qingbin, Zeng Weiqing, Huang Jiabao, Duan Qiaohong, Cao Yunyun
State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China.
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Front Plant Sci. 2022 Aug 25;13:977881. doi: 10.3389/fpls.2022.977881. eCollection 2022.
High temperature negatively affects reproductive process significantly, leading to tremendous losses in crop quality and yield. Zhinengcong (ZNC), a crude extract from the endophytic fungus , has been shown to improve plant growth and resistance to biotic and abiotic stresses. We show here that ZNC can also alleviate heat stress-induced reproductive defects in , such as short-term heat-induced inhibition on pollen viability, germination and tube growth, and long-term heat stress-induced pollen developmental defects. We further demonstrated that ZNC alleviates heat stress by downregulating the expressions of ROS production-related genes, RBOHs, and upregulating antioxidant related genes and the activities of the corresponding enzymes, thus preventing the over accumulation of heat-induced reactive oxygen species (ROS) in anther, pollen grain and pollen tube. Furthermore, spraying application of ZNC onto tomato plants under long-term heat stress promotes fruit and seed bearing in the field. In summary, plant endophytic fungus extract ZNC promotes the reproductive process and yield of tomato plants under heat stress and presents excellent potential in agricultural applications.
高温对生殖过程有显著负面影响，导致作物品质和产量大幅损失。智能聪（ZNC）是一种来自内生真菌的粗提物，已被证明能促进植物生长并提高对生物和非生物胁迫的抗性。我们在此表明，ZNC还可缓解热胁迫诱导的番茄生殖缺陷，如短期热胁迫对花粉活力、萌发和花粉管生长的抑制，以及长期热胁迫诱导的花粉发育缺陷。我们进一步证明，ZNC通过下调活性氧产生相关基因RBOHs的表达，并上调抗氧化相关基因及相应酶的活性，从而减轻热胁迫，防止热诱导的活性氧（ROS）在花药、花粉粒和花粉管中过度积累。此外，在长期热胁迫下对番茄植株喷施ZNC可促进田间结果和结实。总之，植物内生真菌提取物ZNC可促进热胁迫下番茄植株的生殖过程和产量，在农业应用中具有良好潜力。
