持续暴露于低剂量二氧化氯气体对诱导多能干细胞特性的影响。
The effects of continuous exposure to low-dose chlorine dioxide gas on the characteristics of induced pluripotent stem cells.
作者信息
Okawa Ryoma, Sogawa Koushirou, Shiozaki Motoko, Yachiku Kenji, Miura Takanori, Shibata Takashi, Ezoe Sachiko
机构信息
Department of Space Infection Control, Graduate School of Medicine/Faculty of Medicine, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Shonan University of Medical Sciences, 16-10, Kamishinano, Totsuka, Yokohama, Kanagawa 244-0806, Japan.
出版信息
Regen Ther. 2022 Aug 23;21:250-257. doi: 10.1016/j.reth.2022.07.014. eCollection 2022 Dec.
BACKGROUND
Recently, various regenerative therapies have been developed based on induced pluripotent stem (iPS) cells. However, hygienic control strategies have not been established at the manufacturing facilities. We aimed to evaluate the safety and effects of continuous exposure to low-dose chlorine dioxide (ClO) gas on cell fates, and to determine the optimum dose for safe usage of this disinfectant.
METHODS
We cultured an iPS cell line in the absence or presence of various doses of ClO gas. We evaluated cell proliferation, cell death, the maintenance of undifferentiated state, and cell senescence.
RESULTS
We found that iPS cell proliferation was not affected by 0.05 or 0.1 ppmv ClO gas in the atmosphere. Although 0.1 ppmv ClO slightly affected apoptosis, it was not a significant effect. Moreover, neither at 0.05 nor 0.1 ppmv ClO gas significantly affected the characteristics of iPS cells.
DISCUSSION AND CONCLUSION
Continuous exposure to 0.05 or 0.1 ppmv ClO gas did not affect the fate of iPS cells. These results may contribute to the development of new strategies for hygiene control in cell processing facilities.
背景
最近，基于诱导多能干细胞（iPS细胞）开发了各种再生疗法。然而，制造设施尚未建立卫生控制策略。我们旨在评估连续暴露于低剂量二氧化氯（ClO）气体对细胞命运的安全性和影响，并确定这种消毒剂安全使用的最佳剂量。
方法
我们在不存在或存在各种剂量的ClO气体的情况下培养iPS细胞系。我们评估了细胞增殖、细胞死亡、未分化状态的维持和细胞衰老。
结果
我们发现大气中0.05或0.1 ppmv的ClO气体不会影响iPS细胞的增殖。虽然0.1 ppmv的ClO对细胞凋亡有轻微影响，但影响不显著。此外，0.05或0.1 ppmv的ClO气体均未对iPS细胞的特性产生显著影响。
讨论与结论
连续暴露于0.05或0.1 ppmv的ClO气体不会影响iPS细胞的命运。这些结果可能有助于开发细胞处理设施卫生控制的新策略。
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