• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

SEC61G 通过激活 MAPK 信号通路促进宫颈癌增殖。

SEC61G Promotes Cervical Cancer Proliferation by Activating MAPK Signaling Pathway.

机构信息

Department of Obstetrics and Gynecology, Shaanxi Province People's Hospital, Xi'an, 710068 Shaanxi, China.

出版信息

Dis Markers. 2022 Aug 31;2022:7016079. doi: 10.1155/2022/7016079. eCollection 2022.

DOI:10.1155/2022/7016079
PMID:36092956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9452971/
Abstract

OBJECTIVE

The abnormal expression of SEC61G plays an important role in the development of various tumors. This study explored the effects of SEC61G on MAPK signaling pathway and proliferation of cervical cancer (CC) cells.

METHODS

shRNA was used to inhibit the expression of SEC61G and EdU to observe its effect on the proliferation of CC cell SiHa. The effect of SEC61G on invasion was evaluated by Transwell assay. TCGA database was used to analyze the influence of high or low SEC61G expression level on the overall survival of CC patients. Western blot was used to detect the expressions of SEC61G, p-RAF1, Raf1, p-MEK1/2, MEK1/2, and p-ERK1/2 in cells. SiHa cells overexpressing SEC61G (SiHa-SEC61G) and control group (SiHa-mock) were subcutaneously implanted in nude mice. The tumor growth curve was measured at the specified time points between SiHa-SEC61G and SiHa-mock. The inhibitory effect of gefitinib on SEC61G was further evaluated.

RESULTS

In patients with CC, high SEC61G expression predicted poor prognosis. Silencing SEC61G inhibited proliferation and invasion of CC cells in vitro. Overexpression of SEC61G can promote the proliferation and invasion of CC cells in vitro. Meanwhile, overexpression of SEC61G promoted the proliferation of CC xenografts. Knocking down SEC61G can inhibit MAPK signaling pathway. Gefitinib can inhibit CC proliferation and tumor growth by SEC61G.

CONCLUSION

SEC61G is highly expressed in CC and has poor prognosis. Inhibition of SEC61G expression can effectively inhibit the growth and proliferation of human CC cells. The mechanism may be related to the inhibition of MAPK signaling pathway.

摘要

目的

SEC61G 的异常表达在各种肿瘤的发生发展中起着重要作用。本研究探讨了 SEC61G 对 MAPK 信号通路和宫颈癌（CC）细胞增殖的影响。

方法

利用 shRNA 抑制 SEC61G 的表达，并用 EdU 观察其对 CC 细胞 SiHa 增殖的影响。通过 Transwell 实验评估 SEC61G 对侵袭的影响。TCGA 数据库用于分析 SEC61G 高或低表达水平对 CC 患者总生存期的影响。Western blot 用于检测细胞中 SEC61G、p-RAF1、Raf1、p-MEK1/2、MEK1/2 和 p-ERK1/2 的表达。将过表达 SEC61G 的 SiHa 细胞（SiHa-SEC61G）和对照组（SiHa-mock）皮下注射到裸鼠中。在指定时间点测量 SiHa-SEC61G 和 SiHa-mock 之间的肿瘤生长曲线。进一步评估吉非替尼对 SEC61G 的抑制作用。

结果

在 CC 患者中，SEC61G 高表达预示着预后不良。沉默 SEC61G 抑制 CC 细胞的体外增殖和侵袭。SEC61G 的过表达可以促进 CC 细胞的体外增殖和侵袭。同时，SEC61G 的过表达促进了 CC 异种移植的增殖。敲低 SEC61G 可以抑制 MAPK 信号通路。吉非替尼可以通过 SEC61G 抑制 CC 的增殖和肿瘤生长。

结论

SEC61G 在 CC 中高表达，且预后不良。抑制 SEC61G 的表达可以有效抑制人 CC 细胞的生长和增殖。其机制可能与抑制 MAPK 信号通路有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/0412551ca5f9/DM2022-7016079.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/4b9635af8c4a/DM2022-7016079.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/f3c3c39ba7db/DM2022-7016079.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/dc2c0daf27a6/DM2022-7016079.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/eb4cd2666606/DM2022-7016079.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/f71275955e7d/DM2022-7016079.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/0412551ca5f9/DM2022-7016079.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/4b9635af8c4a/DM2022-7016079.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/f3c3c39ba7db/DM2022-7016079.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/dc2c0daf27a6/DM2022-7016079.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/eb4cd2666606/DM2022-7016079.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/f71275955e7d/DM2022-7016079.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bdf/9452971/0412551ca5f9/DM2022-7016079.006.jpg

相似文献

1
SEC61G Promotes Cervical Cancer Proliferation by Activating MAPK Signaling Pathway.SEC61G 通过激活 MAPK 信号通路促进宫颈癌增殖。
Dis Markers. 2022 Aug 31;2022:7016079. doi: 10.1155/2022/7016079. eCollection 2022.
2
SEC61G promotes breast cancer development and metastasis via modulating glycolysis and is transcriptionally regulated by E2F1.SEC61G 通过调节糖酵解促进乳腺癌的发展和转移，并受 E2F1 的转录调控。
Cell Death Dis. 2021 May 27;12(6):550. doi: 10.1038/s41419-021-03797-3.
3
SEC61G is upregulated and required for tumor progression in human kidney cancer.SEC61G 在人类肾癌中上调并促进肿瘤进展。
Mol Med Rep. 2021 Jun;23(6). doi: 10.3892/mmr.2021.12066. Epub 2021 Apr 13.
4
Williams syndrome transcription factor promotes proliferation and invasion of cervical cancer cells by regulating PI3K/Akt signaling pathway.威廉姆斯综合征转录因子通过调节 PI3K/Akt 信号通路促进宫颈癌细胞的增殖和侵袭。
J Obstet Gynaecol Res. 2021 Jul;47(7):2433-2441. doi: 10.1111/jog.14813. Epub 2021 May 24.
5
Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation.SEC61G 在肺腺癌中的预后价值：基于生物信息学和体外验证的综合研究。
BMC Cancer. 2021 Nov 13;21(1):1216. doi: 10.1186/s12885-021-08957-4.
6
[FABP5 promotes cell growth, invasion and metastasis in cervical cancer].[脂肪酸结合蛋白5促进宫颈癌细胞的生长、侵袭和转移]
Zhonghua Zhong Liu Za Zhi. 2019 Mar 23;41(3):200-207. doi: 10.3760/cma.j.issn.0253-3766.2019.03.009.
7
piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.piRNA-14633 通过 METTL14 依赖性 m6A RNA 甲基化促进宫颈癌恶性进展。
J Transl Med. 2022 Jan 29;20(1):51. doi: 10.1186/s12967-022-03257-2.
8
CircFAT1 facilitates cervical cancer malignant progression by regulating ERK1/2 and p38 MAPK pathway through miR-409-3p/CDK8 axis.环状FAT1通过miR-409-3p/CDK8轴调控ERK1/2和p38丝裂原活化蛋白激酶途径促进宫颈癌恶性进展。
Drug Dev Res. 2021 Dec;82(8):1131-1143. doi: 10.1002/ddr.21816. Epub 2021 Apr 5.
9
[Bcl-2 associated athanogene 3 affects the epithelial-mesenchymal transition in human cervical cancer].[Bcl-2相关生原基因3影响人宫颈癌中的上皮-间质转化]
Zhonghua Fu Chan Ke Za Zhi. 2017 Aug 25;52(8):551-557. doi: 10.3760/cma.j.issn.0529-567X.2017.08.009.
10
Aberrantly enhanced melanoma-associated antigen (MAGE)-A3 expression facilitates cervical cancer cell proliferation and metastasis via actuating Wnt signaling pathway.异常增强的黑色素瘤相关抗原（MAGE）-A3 表达通过激活 Wnt 信号通路促进宫颈癌癌细胞的增殖和转移。
Biomed Pharmacother. 2020 Feb;122:109710. doi: 10.1016/j.biopha.2019.109710. Epub 2019 Dec 30.

引用本文的文献

1
SEC61G promotes colorectal cancer progression by regulating cytosolic Ca concentration.SEC61G通过调节胞质钙浓度促进结直肠癌进展。
J Gastroenterol. 2025 May 25. doi: 10.1007/s00535-025-02259-3.
2
An original aneuploidy-related gene model for predicting lung adenocarcinoma survival and guiding therapy.一种用于预测肺腺癌生存和指导治疗的原始非整倍体相关基因模型。
Sci Rep. 2024 Apr 7;14(1):8135. doi: 10.1038/s41598-024-58020-y.
3
SEC61 translocon subunit gamma enhances low-dose cisplatin-induced cancer-stem cell properties of head and neck squamous cell carcinoma via enhancing Ca-mediated autophagy.

本文引用的文献

1
HPV Vaccination and the Risk of Invasive Cervical Cancer.HPV 疫苗接种与浸润性宫颈癌风险。
N Engl J Med. 2020 Oct 1;383(14):1340-1348. doi: 10.1056/NEJMoa1917338.
2
Targeting MAPK Signaling in Cancer: Mechanisms of Drug Resistance and Sensitivity.靶向癌症中的 MAPK 信号通路：药物耐药性和敏感性的机制。
Int J Mol Sci. 2020 Feb 7;21(3):1102. doi: 10.3390/ijms21031102.
3
Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling.Musashi2 通过 ZEB1-ERK/MAPK 信号促进胰腺癌中 EGF 诱导的 EMT。
SEC61转运体亚基γ通过增强钙介导的自噬增强低剂量顺铂诱导的头颈部鳞状细胞癌的癌症干细胞特性。
J Dent Sci. 2023 Oct;18(4):1685-1692. doi: 10.1016/j.jds.2023.01.024. Epub 2023 Feb 4.
J Exp Clin Cancer Res. 2020 Jan 17;39(1):16. doi: 10.1186/s13046-020-1521-4.
4
Estimates of incidence and mortality of cervical cancer in 2018: a worldwide analysis.2018 年宫颈癌发病率和死亡率的估计：全球分析。
Lancet Glob Health. 2020 Feb;8(2):e191-e203. doi: 10.1016/S2214-109X(19)30482-6. Epub 2019 Dec 4.
5
A Comprehensive Review on MAPK: A Promising Therapeutic Target in Cancer.丝裂原活化蛋白激酶（MAPK）的全面综述：癌症中一个有前景的治疗靶点
Cancers (Basel). 2019 Oct 22;11(10):1618. doi: 10.3390/cancers11101618.
6
Key Molecular Events in Cervical Cancer Development.宫颈癌发展中的关键分子事件。
Medicina (Kaunas). 2019 Jul 17;55(7):384. doi: 10.3390/medicina55070384.
7
Identification of SEC61G as a Novel Prognostic Marker for Predicting Survival and Response to Therapies in Patients with Glioblastoma.鉴定 SEC61G 作为胶质母细胞瘤患者生存和治疗反应的新型预后标志物。
Med Sci Monit. 2019 May 16;25:3624-3635. doi: 10.12659/MSM.916648.
8
Systemic therapy in cervical cancer: 30 years in review.宫颈癌的全身治疗：30 年回顾。
Crit Rev Oncol Hematol. 2019 May;137:9-17. doi: 10.1016/j.critrevonc.2019.02.009. Epub 2019 Feb 28.
9
Cervical Cancer: An Overview of Pathophysiology and Management.宫颈癌：病理生理学与管理概述。
Semin Oncol Nurs. 2019 Apr;35(2):166-174. doi: 10.1016/j.soncn.2019.02.003. Epub 2019 Mar 14.
10
RAS/MAPK signaling functions in oxidative stress, DNA damage response and cancer progression.RAS/MAPK信号传导在氧化应激、DNA损伤反应和癌症进展中发挥作用。
J Cell Physiol. 2019 Sep;234(9):14951-14965. doi: 10.1002/jcp.28334. Epub 2019 Feb 27.