Department of Environmental Ecological Engineering, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing, China.
School of Marine Sciences, Nanjing University of Information Science and Technology, Nanjing, China.
Front Cell Infect Microbiol. 2022 Aug 26;12:904284. doi: 10.3389/fcimb.2022.904284. eCollection 2022.
Alterations in gut microbiota might contribute to uremic toxicity and immune dysregulation in patients with end-stage renal disease. Hemodialysis patients are prone to infection and higher mortality following sepsis. The virulence factors in the gut metagenome have not been well studied in hemodialysis patients, which could be employed by microorganisms to successfully thrive and flourish in their hosts. In this study, we performed shotgun metagenomics sequencing on fecal DNA collected from 16 control subjects and 24 hemodialysis patients. Our analysis shows that a number of microbial species, metabolic pathways, antibiotic resistance, and virulence factors were significantly altered in hemodialysis patients compared with controls. In particular, erythromycin resistance methylase, pyridoxamine 5-phosphate oxidase, and streptothricin-acetyl-transferase were significantly increased in hemodialysis patients. The findings in our study laid a valuable foundation to further elucidate the causative role of virulence factors in predisposing HD patients to infection and to develop treatment strategies to reduce the genetic capacities of antibiotic resistance and virulence factors in HD patients.
肠道微生物群的改变可能导致终末期肾病患者的尿毒症毒性和免疫失调。血液透析患者在发生脓毒症后容易感染和死亡率更高。在血液透析患者中，尚未很好地研究肠道宏基因组中的毒力因子，这些因子可能被微生物用来在宿主中成功地生存和繁殖。在这项研究中，我们对 16 名对照者和 24 名血液透析患者的粪便 DNA 进行了鸟枪法宏基因组测序。我们的分析表明，与对照组相比，血液透析患者的许多微生物物种、代谢途径、抗生素耐药性和毒力因子发生了显著改变。特别是，血液透析患者中红霉素耐药甲基酶、吡哆醇 5-磷酸氧化酶和链霉菌乙酰转移酶显著增加。我们的研究结果为进一步阐明毒力因子在易感染血液透析患者中的致病作用奠定了有价值的基础，并为开发治疗策略以降低血液透析患者抗生素耐药性和毒力因子的遗传能力提供了依据。
