Anderson G J, Kumar S
FEBS Lett. 1987 Aug 17;220(2):323-6. doi: 10.1016/0014-5793(87)80839-6.
An assay for the transacylation reaction catalyzed by fatty acid synthase was developed which does not require model substrates or labelled acyl-derivatives of CoA. It involves the transfer of the acyl group from unlabelled CoA to [3H]CoA. This assay shows the occurrence of transacylation at a relatively high rate with a variety of substrates that the enzyme is able to utilize. The activity is unaffected by dissociation of the enzyme or modification by iodoacetamide or 2-chloroacetyl-CoA.
开发了一种用于检测脂肪酸合酶催化的转酰基反应的方法，该方法不需要模型底物或辅酶A的标记酰基衍生物。它涉及将酰基从未标记的辅酶A转移到[3H]辅酶A上。该检测方法表明，该酶能够利用的多种底物都能以相对较高的速率发生转酰基反应。该活性不受酶的解离或碘乙酰胺或2-氯乙酰辅酶A修饰的影响。
