Mikkelsen J, Højrup P, Hansen H F, Hansen J K, Knudsen J
Biochem J. 1985 May 1;227(3):981-5. doi: 10.1042/bj2270981.
Competitive binding experiments with malonyl-CoA and [1-14C]acetyl-CoA, [1-14C]butyryl-CoA or [1-14C]decanoyl-CoA indicate that all these substrates are transferred to lactating-goat mammary-gland fatty acid synthetase by the same transferase. Isolation and determination of the amino acid sequence of [1-14C]decanoyl-labelled CNBr-cleavage peptide from the decanoyltransferase site showed that this transferase is identical with the acetyl/malonyltransferase.
用丙二酰辅酶A与[1-¹⁴C]乙酰辅酶A、[1-¹⁴C]丁酰辅酶A或[1-¹⁴C]癸酰辅酶A进行的竞争性结合实验表明,所有这些底物都是通过同一转移酶转移到泌乳山羊乳腺脂肪酸合成酶上的。从癸酰转移酶位点分离并测定[1-¹⁴C]癸酰标记的溴化氰裂解肽的氨基酸序列表明,该转移酶与乙酰/丙二酰转移酶相同。