Mezl V A, Cutcliffe N
Int J Biochem. 1987;19(6):523-9. doi: 10.1016/0020-711x(87)90136-4.
The amount of RNA obtained from rabbit reticulocyte membrane-bound ribosomes by direct phenol extraction of washed membranes was inversely related to the hematocrit of the animals. Translation of the RNA in the reticulocyte translation system showed that the Mr = 30,000 protein reported to be a marker of membrane polysomes was also made by an endogenous mRNA in this translation system. Analyses of the translation products made in the wheat germ system on Triton X-100 acid urea gels show that membrane RNAs display a characteristic alpha- to beta-globin ratio of 0.77 which differentiates them from RNAs prepared from cytoplasmic polysomes and from the postpolysomal supernatant. These results show that free and membrane-bound ribosomes can be distinguished by the main protein that they produce.
通过对洗涤过的膜进行直接苯酚抽提，从兔网织红细胞膜结合核糖体获得的RNA量与动物的血细胞比容呈负相关。在网织红细胞翻译系统中对该RNA进行翻译显示，据报道为膜多核糖体标志物的分子量为30,000的蛋白质也由该翻译系统中的内源性mRNA合成。在Triton X-100酸性尿素凝胶上对小麦胚芽系统中产生的翻译产物进行分析表明，膜RNA显示出特征性的α-珠蛋白与β-珠蛋白比例为0.77，这使其与从细胞质多核糖体和多核糖体后上清液制备的RNA区分开来。这些结果表明，游离核糖体和膜结合核糖体可以通过它们产生的主要蛋白质来区分。
