Crkvenjakov R, Cusić S, Ivanović I, Glisin V
Eur J Biochem. 1976 Dec;71(1):85-91. doi: 10.1111/j.1432-1033.1976.tb11092.x.
Globin mRNAs were isolated from circulating reticulocytes both from rats carrying a homozygous, recessive mutation causing a severe thalassemia-like syndrome and from normal rats. After first identifying the rat globin chains as alpha or beta chains, the translational products primed by both polysomal and nonpolysomal mRNAs in wheat germ 30000 x g supernatant were analyzed: the ratio of alpha to beta globin mRNAs found in polysomes isolated from mutant rats is identical to the ratio of their products synthesized in vivo while the ratio of these mRNAs is quite different in the nonpolysomal fraction, the latter being enriched in alpha globin mRNA. No difference is found in the ratio of alpha and beta globin mRNAs in the polysomal and nonpolysomal RNA isolated from normal rats, both being identical to the ratio of their products synthesized in vivo. One third of the total amount of mRNA found in mutant cells is not in polysomes as compared to only 6 percent for the mRNA from normal lysates. These results suggest that a translational control mechanism is involved although the decreased globin synthesis in b/b anemia can not be fully accounted for by its operation.
从患有导致严重地中海贫血样综合征的纯合隐性突变大鼠以及正常大鼠的循环网织红细胞中分离出珠蛋白mRNA。在首先将大鼠珠蛋白链鉴定为α链或β链后，分析了在小麦胚芽30000×g上清液中由多聚核糖体和非多聚核糖体mRNA引发的翻译产物：从突变大鼠分离的多聚核糖体中发现的α珠蛋白mRNA与β珠蛋白mRNA的比例与其在体内合成产物的比例相同，而这些mRNA在非多聚核糖体部分的比例则有很大差异，后者富含α珠蛋白mRNA。从正常大鼠分离的多聚核糖体和非多聚核糖体RNA中，α珠蛋白mRNA与β珠蛋白mRNA的比例没有差异，两者均与它们在体内合成产物的比例相同。与正常裂解物的mRNA仅6%相比，突变细胞中发现的mRNA总量的三分之一不在多聚核糖体中。这些结果表明涉及一种翻译控制机制，尽管b/b贫血中珠蛋白合成的减少不能完全由其作用来解释。
