Traslational Research Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico.
Department of Surgery, Faculty of Medicine, The National Autonomous University of Mexico (UNAM), Mexico City, Mexico; Hepato Pancreato and Biliary Clinic, Department of General Surgery, "Hospital General de Mexico", Dr. Eduardo Liceaga. Mexico City, Mexico; Obesity and Digestive Diseases Unit, Medica Sur Clinic & Foundation, Mexico City, Mexico.
Ann Hepatol. 2022 Nov-Dec;27(6):100756. doi: 10.1016/j.aohep.2022.100756. Epub 2022 Sep 10.
Metabolic-associated fatty liver disease (MAFLD) is defined by steatosis in more than 5% of hepatocytes without other liver diseases. Patients with this disease can progress to multiple stages like liver fibrosis, cirrhosis, and hepatocellular carcinoma. miRNAs are single-stranded molecules that regulate metabolic homeostasis; their differential expression postulates them as potential circulating biomarkers for MAFLD. Previous research reported that hsa-miR-140-5p, hsa-miR-148-5p, and hsa-miR-122-3p have a differential expression in patients with MAFLD. This study aimed to investigate the correlation between liver hsa-miR-140-5p, hsa-miR-148-5p, and hsa-miR-122-3p and serum biomarkers CK-18, APOB, IL-6, IL-32, and TNF-α in patients with MAFLD compared with control patients.
A cross-sectional study was carried out with 16 patients of both sexes, aged between 18-60 years, to determine the association between the levels of hsa-miR-140-5p, hsa-miR-148-5p, and hsa-miR-122-3p with MAFLD in liver biopsies of patients who underwent laparoscopic cholecystectomy.
Twelve patients presented MAFLD, four without hepatic steatosis. Circulating levels of CK-18 showed a significant difference in patients with MAFLD, and a strong correlation was found between hsa-miR-122-3p, hsa-miR-140-5p, and hsa-miR-148b-5p versus the CAP value.
There is a correlation between elevated tissue expression of hsa-miR-122-3p, hsa-miR-140-5p, and hsa-miR-148b-3p with plasma levels of CK-18 in patients with simple steatosis compared with patients without the disease.
代谢相关脂肪性肝病（MAFLD）定义为超过 5%的肝细胞发生脂肪变性，且无其他肝病。此类患者可进展至肝纤维化、肝硬化和肝细胞癌等多个阶段。miRNAs 是调节代谢稳态的单链分子，其差异性表达使其成为 MAFLD 潜在的循环生物标志物。既往研究报道 hsa-miR-140-5p、hsa-miR-148-5p 和 hsa-miR-122-3p 在 MAFLD 患者中存在差异性表达。本研究旨在探讨 MAFLD 患者肝组织 hsa-miR-140-5p、hsa-miR-148-5p 和 hsa-miR-122-3p 与对照组患者血清生物标志物 CK-18、APOB、IL-6、IL-32 和 TNF-α 之间的相关性。
采用横断面研究方法，纳入 16 例 18-60 岁的男女患者，通过腹腔镜胆囊切除术获取肝组织活检，以确定 hsa-miR-140-5p、hsa-miR-148-5p 和 hsa-miR-122-3p 水平与 MAFLD 的相关性。
12 例患者存在 MAFLD，4 例患者无肝脂肪变性。MAFLD 患者的 CK-18 循环水平存在显著差异，且 hsa-miR-122-3p、hsa-miR-140-5p 和 hsa-miR-148b-5p 与 CAP 值呈强相关性。
与无该病的患者相比，单纯性脂肪变性患者组织 hsa-miR-122-3p、hsa-miR-140-5p 和 hsa-miR-148b-3p 的表达升高与 CK-18 血浆水平相关。
