非酒精性脂肪性肝炎中环状RNA的表达谱及N6-甲基腺嘌呤修饰分析

Expression profile and N6-methyadenosine modification of circular RNA analysis in MAFLD.

作者信息

Zheng Mengyao, Cun Dongyun, He Haiyu, Xie Xuancheng, Lei Hongtao, Fu Wen, Tai Wenlin, Yang Jinhui

机构信息

Department of Gastroenterology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China.

Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China.

出版信息

BMC Gastroenterol. 2025 Mar 11;25(1):162. doi: 10.1186/s12876-025-03722-4.

DOI:10.1186/s12876-025-03722-4

PMID:40069595

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11899672/

Abstract

BACKGROUND

To analyze the expression patterns of circRNAs in metabolic associated fatty liver disease (MAFLD) and the regulation of mA methylation on those circRNAs.

METHODS

The expression profile of CircRNA in MAFLD and normal control liver tissues was analyzed by microarray. Predict the potential mA sites of the differentially expression circRNAs (DECs) via the SRAMP website. The biological functions and molecular interactions of DECs were analyzed by GO and KEGG analyses. The selected DECs were verified by MeRIP-qPCR and RT-qPCR.

RESULTS

There were 59 DECs in MAFLD liver tissues compared with normal control liver tissues. We found that mA sites with high or very high confidence were present in 39 of these DECs. Four randomly selected DECs were validated by RT-qPCR, hsa-MLIP_0004, hsa-CHD2_0084 and hsa-FOXP1_0001 matched well with the microarray results. mA qualification of them were conducted by MeRIP-qPCR, the mA methylation levels are significantly different between the MAFLD and NC groups.

CONCLUSION

In MAFLD, the dysregulated expression of circRNAs may be influenced by mA modifications. This study provides preliminary evidence suggesting that mA-mediated regulation of circRNAs could play a role in the progression of MAFLD, laying the foundation for exploring the epigenetic regulation of circRNAs in MAFLD and offering potential avenues for future diagnostic and therapeutic strategies.

TRIAL REGISTRATION

Not applicable.

摘要

背景

分析环状RNA（circRNAs）在代谢相关脂肪性肝病（MAFLD）中的表达模式以及m⁶A甲基化对这些circRNAs的调控作用。

方法

通过微阵列分析MAFLD和正常对照肝脏组织中circRNA的表达谱。通过SRAMP网站预测差异表达circRNAs（DECs）的潜在m⁶A位点。通过基因本体（GO）和京都基因与基因组百科全书（KEGG）分析来分析DECs的生物学功能和分子相互作用。通过甲基化RNA免疫沉淀定量PCR（MeRIP-qPCR）和逆转录定量PCR（RT-qPCR）对所选的DECs进行验证。

结果

与正常对照肝脏组织相比，MAFLD肝脏组织中有59个DECs。我们发现其中39个DECs存在高置信度或非常高置信度的m⁶A位点。通过RT-qPCR对随机选择的4个DECs进行验证，hsa-MLIP_0004、hsa-CHD2_0084和hsa-FOXP1_0001与微阵列结果匹配良好。通过MeRIP-qPCR对它们进行m⁶A鉴定，MAFLD组和正常对照组之间的m⁶A甲基化水平存在显著差异。