Sharma B S, Elias A N
Gen Pharmacol. 1987;18(4):449-53. doi: 10.1016/0306-3623(87)90107-8.
Methimazole (MM), an antithyroid drug, was examined for its ability to modulate immune functions. The increase in [3H]thymidine incorporation in plant mitogen-stimulated lymphocytes and augmentation of NK cell activity by MM were used as a measure of the immunomodulatory activity of MM. The results demonstrated that lymphocytes from 5 different donors had a mean increase of 632% of [3H]thymidine incorporation in the presence of MM at a concentration of 114.2 micrograms/ml (P less than 0.005). Lymphocyte response to PHA, Con A or PWM was potentiated by 27 to 482% in the presence of MM. The MM mediated increase in lymphocyte proliferation was more obvious in cases where the mitogenic stimulation was low. Lymphocyte cytotoxicity to both K562 cells and malignant B cells was also augmented when lymphocytes were cultured in the presence of MM. A maximal effect was observed when lymphocytes were treated with a concentration of 20 micrograms/ml of MM for 12-16 hr. Maximum augmentation was usually exerted when the NK cell activity of cultured lymphocytes was low. This was particularly seen with lymphocyte from cultured B cell leukemia (3163).
甲巯咪唑（MM），一种抗甲状腺药物，对其调节免疫功能的能力进行了研究。MM对植物有丝分裂原刺激的淋巴细胞中[3H]胸腺嘧啶核苷掺入的增加以及NK细胞活性的增强被用作衡量MM免疫调节活性的指标。结果表明，来自5个不同供体的淋巴细胞在浓度为114.2微克/毫升的MM存在下，[3H]胸腺嘧啶核苷掺入平均增加了632%（P<0.005）。在MM存在下，淋巴细胞对PHA、Con A或PWM的反应增强了27%至482%。在有丝分裂原刺激较低的情况下，MM介导的淋巴细胞增殖增加更为明显。当淋巴细胞在MM存在下培养时，其对K562细胞和恶性B细胞的细胞毒性也增强。当用浓度为20微克/毫升的MM处理淋巴细胞12至16小时时观察到最大效应。当培养的淋巴细胞的NK细胞活性较低时，通常会产生最大增强作用。这在培养的B细胞白血病（3163）的淋巴细胞中尤为明显。
