Hema S K, Karmakar Aparajita, Das Raunak Kumar, Srivastava Priyanka
School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India.
Centre for Biomaterials and Molecular Theranostics, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India.
Heliyon. 2022 Aug 24;8(9):e10397. doi: 10.1016/j.heliyon.2022.e10397. eCollection 2022 Sep.
Multiple emulsions are thermodynamically stable systems that mark applications in various fields including drug delivery systems. They allow enhanced availability of drugs, greater absorption, and present reduced toxicity, among other desirable properties. In this work, we aimed to formulate a unique double emulsion (O1/W + W1/O2/W/W) with three bioactive components viz. oil, oil and crystalline Three surfactants with different HLB values viz. Tween-20, Tween-80 and Triton X-100 were used for the emulsification process. The method followed was simple as compared to current methods employed for formulating multiple emulsions. Formulation was characterized using techniques of bright field microscopy, Dynamic Light Scattering (DLS), High-Resolution Transmission Electron Microscopy (HR-TEM) and Fourier-transform infrared spectroscopy (FTIR). Image processing tools were also used to characterize the formulation, which reliably cross-verified the observations from conventional characterization techniques. The potency of individual components of emulsion was compared with the prepared double emulsion model by testing the activity on two pathologically relevant bacterial strains: (FN) and (PG).
多重乳液是热力学稳定体系，在包括药物递送系统在内的各个领域都有应用。它们具有提高药物利用率、增强吸收以及降低毒性等其他理想特性。在这项工作中，我们旨在制备一种独特的双重乳液（O1/W + W1/O2/W/W），其中包含三种生物活性成分，即油、油和晶体。使用了三种具有不同亲水亲油平衡（HLB）值的表面活性剂，即吐温 - 20、吐温 - 80和曲拉通X - 100进行乳化过程。与目前用于制备多重乳液的方法相比，所采用的方法很简单。使用明场显微镜、动态光散射（DLS）、高分辨率透射电子显微镜（HR - TEM）和傅里叶变换红外光谱（FTIR）技术对制剂进行表征。还使用图像处理工具对制剂进行表征，从而可靠地交叉验证了来自传统表征技术的观察结果。通过在两种病理相关细菌菌株：（FN）和（PG）上测试活性，将乳液中各个成分的效力与制备的双重乳液模型进行了比较。
