Department of Pediatric Dentistry, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
Department of Periodontology, Hacettepe University, Ankara, Turkey.
Pediatr Pulmonol. 2022 Nov;57(11):2839-2846. doi: 10.1002/ppul.26145. Epub 2022 Sep 19.
To evaluate the caries status of the Cystic fibrosis (CF) children and adolescents with the comparation of some biochemical markers, secretory-immunoglobulin-A (sIgA), and antimicrobial peptides in the saliva.
In this cross-sectional descriptive study, the approval Ethics Board was obtained. Unstimulated saliva samples were collected from CF and healthy control children (non-CF) patients. Both groups underwent the same dental and periodontal evaluation scheme of the assessment. Human beta defensin (HBD1), human alpha defensin (HNP-1), cathelicidin (LL-37), sIgA in saliva were evaluated by enzyme-linked immunoassay method. A general biochemical analysis was performed. Statistical analysis was performed by using Statistical Package for the Social Sciences Version 20.0 (SPSS Inc.).
A total of 21 (9 male, 12 female) CF and 23 (11 male, 12 female) control patients were participated with the mean age of 10.17 ± 3.38 and 9.52 ± 2.15 years, respectively. In control children, DMFT/S (decayed-missing-filled-tooth/surface-in-permanent-dentition), dmft/s (decayed-missing-filled-tooth/surface-in-primary-dentition) values were higher; DT (decayed-tooth in permanent dentition), ft (filled-tooth in primary dentition) and plaque index values were statistically significantly higher (p = 0.042, p = 0.005, p = 0.038, respectively) than CF patients. Bicarbonate was higher in control group; sodium, chloride, and total protein were higher in CF group; magnesium, calcium and phosphate levels were similar in each group (p > 0.05). Alpha and beta defensin-1 levels in control group was statistically significantly higher (p = 0.037 and p = 0.020, respectively), while LL37 and sIgA were not statistically significantly higher (p > 0.05) than CF group.
Children with CF had lower caries in permanent teeth, filling in primary teeth, and an altered salivary biomarker profile, especially in HNB1, HNP1. Therefore, it is important to conduct periodic oral-dental controls among CF patients during their childhood.
通过比较唾液中的分泌型免疫球蛋白 A（sIgA）和抗菌肽等一些生化标志物，评估囊性纤维化（CF）儿童和青少年的龋齿状况。
本研究为横断面描述性研究，获得了伦理委员会的批准。采集 CF 组和健康对照组（非 CF 组）儿童患者的非刺激性唾液样本。两组均接受相同的牙齿和牙周评估方案。采用酶联免疫吸附法检测唾液中人β防御素（HBD1）、人α防御素（HNP-1）、抗菌肽（LL-37）和 sIgA。进行一般生化分析。采用 SPSS 20.0 版统计软件进行统计学分析。
共纳入 21 名（男 9 名，女 12 名）CF 组和 23 名（男 11 名，女 12 名）对照组患者，平均年龄分别为 10.17±3.38 岁和 9.52±2.15 岁。在对照组儿童中，DMFT/S（恒牙龋失补牙面数）、dmft/s（乳牙龋失补牙面数）值较高；DT（恒牙龋失补牙数）、ft（乳牙龋失补牙数）和菌斑指数值均显著高于 CF 组（p=0.042、p=0.005、p=0.038）。对照组的碳酸氢盐水平较高，而 CF 组的钠、氯和总蛋白水平较高；各组镁、钙和磷水平相似（p＞0.05）。对照组的α和β防御素-1 水平显著高于 CF 组（p=0.037 和 p=0.020），而 LL37 和 sIgA 水平无统计学差异（p＞0.05）。
CF 儿童的恒牙龋齿、乳牙补牙和唾液生物标志物谱发生改变，尤其是 HNB1 和 HNP1 水平较低。因此，在儿童时期，对 CF 患者进行定期口腔牙科检查非常重要。
